The effect of light sensitizer localization on the stability of indocyanine green liposomes
| Autor: | Tatu Lajunen, Arto Urtti, Alex Bunker, Teemu Ruoslahti, Tomasz Róg, Ossi Korhonen, Marika Ruponen, Niklas G. Johansson, Danny Wilbie, Riikka Nurmi |
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| Přispěvatelé: | Tampere University, Physics, Pharmaceutical Nanotechnology, Division of Pharmaceutical Biosciences, Drug Delivery Unit, Faculty of Pharmacy, Pharmaceutical Design and Discovery group, Division of Pharmaceutical Chemistry and Technology, Department of Physics, Doctoral Programme in Materials Research and Nanosciences, Drug Research Program, Pharmaceutical biophysics group, Doctoral Programme in Drug Research, Drug Delivery |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Light
genetic structures Swine 116 Chemical sciences Pharmaceutical Science 02 engineering and technology 01 natural sciences chemistry.chemical_compound Coloring Agents Lipid bilayer IN-VIVO Liposome Chemistry Bilayer Triggered release RETINAL-PIGMENT EPITHELIUM Fluoresceins 021001 nanoscience & nanotechnology MOLECULAR-DYNAMICS SIMULATION Indocyanine green 317 Pharmacy Drug delivery 0210 nano-technology Stability Polyethylene glycol FLUORESCENCE PROPERTIES Drug delivery system Molecular Dynamics Simulation 010402 general chemistry 114 Physical sciences Amphiphile PEG ratio Animals Humans PHOTOSENSITIVE LIPOSOMES ATOM FORCE-FIELD Fluorescent Dyes Photolysis DRUG-DELIVERY SYSTEMS eye diseases 0104 chemical sciences Drug Liberation PHOTOINITIATED DESTABILIZATION Delayed-Action Preparations Liposomes GOLD NANOPARTICLES Biophysics POLYETHYLENE-GLYCOL DERIVATIVES |
| Popis: | Light triggered drug delivery systems offer attractive possibilities for sophisticated therapy, providing both temporal and spatial control of drug release. We have developed light triggered liposomes with clinically approved indocyanine green (ICG) as the light sensitizing compound. Amphiphilic ICG can be localized in different compartments of the liposomes, but the effect of its presence, on both triggered release and long term stability, has not been studied. In this work, we report that ICG localization has a significant effect on the properties of the liposomes. Polyethylene glycol (PEG) coating of the liposomes leads to binding and stabilization of the ICG molecules on the surface of the lipid bilayer. This formulation showed both good storage stability in buffer solution (at +4-37 degrees C) and adequate stability in serum and vitreous (at +37 degrees C). The combination of ICG within the lipid bilayer and PEG coating lead to poor stability at elevated temperatures of +22 degrees C and +37 degrees C. The mechanisms of the increased instability due to ICG insertion in the lipid bilayer was elucidated with molecular dynamics simulations. Significant PEG insertion into the bilayer was induced in the presence of ICG in the lipid bilayer. Finally, feasibility of freeze-drying as a long term storage method for the ICG liposomes was demonstrated. Overall, this is the first detailed study on the interactions of lipid bilayer, light sensitizer (ICG) and PEG coating on the liposome stability. The localization of the light triggering agent significantly alters the structure of the liposomes and it is important to consider these aspects in triggered drug delivery system design. |
| Databáze: | OpenAIRE |
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