Production of transgenic cattle by somatic cell nuclear transfer (SCNT) with the human granulocyte colony-stimulation factor (hG-CSF)
| Autor: | Ligiane O Leme, B. D. M. Silva, Bruno Leal Pastor de Carvalho, R. V. Sousa, Andrielle Thainar Mendes Cunha, Margot Alves Nunes Dode, E. O. Melo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Veterinary (miscellaneous) Transgene Granulocyte Biology Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences 0302 clinical medicine Immune system medicine lcsh:SF1-1100 Lipofection Cloning Ecology Research Transfection Leukopenia Cell biology 030104 developmental biology medicine.anatomical_structure Genetically modified organisms Somatic cell nuclear transfer Animal Science and Zoology lcsh:Animal culture Expression cassette Bone marrow 030215 immunology Food Science Biotechnology |
| Zdroj: | Journal of Animal Science and Technology Journal of Animal Science and Technology, Vol 61, Iss 2, Pp 61-68 (2019) |
| ISSN: | 2055-0391 |
| Popis: | The hG-CSF (human Granulocyte Colony-Stimulating Factor) is a growth and stimulation factor capable of inducing the proliferation of bone marrow cells, several types of leukocytes, among other hematopoietic tissue cells. hG-CSF is used in used to treat anomalies that reder a small number of circulating white blood cells, which may compromise the immune defenses of the affected person. For these reasons, the production of hG-CSF in a bioreactor system using the mammary gland of genetic modified animals is a possibility of adding value to the bovine genetic material and reducing the costs of hG-CSF production in pharmaceutical industry. In this study, we aimed the production of transgenic hG-CSF bovine through the lipofection of bovine primary fibroblasts with an hG-CSF expression cassette and cloning these fibroblasts by the somatic cell nuclear transfer (SCNT) technique. The bovine fibroblasts transfected with the hG-CSF cassette presented a stable insertion of this construct into their genome and were efficiently synchronized to G0/G1 cell cycle stage. The transgenic fibroblasts were cloned by SCNT and produced 103 transferred embryos and 2 pregnancies, one of which reached 7 months of gestation. |
| Databáze: | OpenAIRE |
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