Proteomic profile of preeclampsia in the first trimester of pregnancy
| Autor: | Rodolfo Amador Salazar-Ybarra, Santos Guzmán-López, Víctor Torres-de-la-Cruz, Guillermo Jacobo-Baca, Rodrigo Enrique Elizondo-Omaña, Abel Guzmán-López, Laura Elia Martínez-de-Villarreal, Miguel Ángel Vázquez-Barragán |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
0301 basic medicine medicine.medical_specialty Pregnancy Trimester Third 030204 cardiovascular system & hematology Clinical onset Preeclampsia 03 medical and health sciences 0302 clinical medicine Pre-Eclampsia Pregnancy Tandem Mass Spectrometry medicine Humans Proteomic Profile Serine-Arginine Splicing Factors Obstetrics business.industry Infant Newborn Obstetrics and Gynecology medicine.disease Neonatal morbidity Pregnancy Trimester First First trimester 030104 developmental biology Case-Control Studies Pediatrics Perinatology and Child Health Biomarker (medicine) Female business Biomarkers |
| Zdroj: | The Journal of Maternal-Fetal & Neonatal Medicine. 35:3446-3452 |
| ISSN: | 1476-4954 1476-7058 |
| Popis: | Preeclampsia (PE) is a leading cause of pregnancy-associated maternal and neonatal morbidity and mortality. Detection of patients at risk before the clinical onset of PE is a priority. Proteomics have become a valuable tool for the discovery of new biomarkers; however, the understanding of the underlying mechanism is necessary. The aim of the study was to determine differences between proteomic serum profiles of PE and normotensive pregnancies using quantitative and qualitative approaches.Serum samples from pregnant women were taken at 10-12 weeks of gestation with follow-up to determine PE development. Samples were analyzed using nano 2-D liquid chromatography UPLC and qTOF-MS/MS.A total of 136 women were recruited, of which eight (5.9%) developed PE, and eight normotensive were randomly selected as a control group for comparison. A different profile was obtained between groups. Nine proteins showed quantitative differences with fold-change over 1.5: PRRC2C (217.02), HEATR5A (179.46), ATP6 (162.38), PRRC2B (83.09), RBM25 (5.36), NUP205 (3.38), HLA-I (2.27), ZC3H13 (2.15), and SREK1 (1.66); and two under 0.66: Importin-4 (0.55) and Cytochrome b (0.26). Using bilateral Fisher's exact test for the qualitative approach, LRRK1 had statistical significance (The found proteins have plausibility with the early pathophysiological events that have been associated with this pathology. Further studies should be performed to confirm these findings and elucidate their specific roles. |
| Databáze: | OpenAIRE |
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