Asiaticoside induces human collagen I synthesis through TGFbeta receptor I kinase (TbetaRI kinase)-independent Smad signaling
| Autor: | Junho Park, Young-Ji Kim, Jinil Park, Deokhoon Park, Ji-Eun Kim, Eunsun Jung, Jongsung Lee, Sungtaek Hong, Yeong Shik Kim, Chang-Gu Hyun |
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| Rok vydání: | 2006 |
| Předmět: |
Blotting
Western Receptor Transforming Growth Factor-beta Type I Pharmaceutical Science SMAD Biology Protein Serine-Threonine Kinases Collagen Type I Analytical Chemistry Dermal fibroblast Centella Keratolytic Agents Drug Discovery Mothers against decapentaplegic homolog 4 Humans Smad3 Protein Smad4 Protein Pharmacology Kinase Plant Extracts Organic Chemistry Transforming growth factor beta Fibroblasts Triterpenes Cell biology Skin Aging Complementary and alternative medicine Biochemistry biology.protein Molecular Medicine Phosphorylation Signal transduction Receptors Transforming Growth Factor beta Type I collagen Phytotherapy Signal Transduction |
| Zdroj: | Planta medica. 72(4) |
| ISSN: | 0032-0943 |
| Popis: | Skin aging appears to be principally related to a decrease in the levels of type I collagen, the primary component of the skin dermis. Asiaticoside, a saponin component isolated from Centella asiatica, has been shown to induce type I collagen synthesis in human dermal fibroblast cells. However, the mechanism underlying asiaticoside-induced type I collagen synthesis, especially at a molecular level, remains only partially understood. In this study, we have attempted to characterize the action mechanism of asiaticoside in type I collagen synthesis. Asiaticoside was determined to induce the phosphorylation of both Smad 2 and Smad 3. In addition, we detected the asiaticoside-induced binding of Smad 3 and Smad 4. In a consistent result, the nuclear translocation of the Smad 3 and Smad 4 complex was induced via treatment with asiaticoside, pointing to the involvement of asiaticoside in Smad signaling. In addition, SB431542, an inhibitor of the TGFbeta receptor I (TbetaRI) kinase, which is known to be an activator of the Smad pathway, was not found to inhibit both Smad 2 phosphorylation and Type 1 collagen synthesis induced by asiaticoside. Therefore, our results show that asiaticoside can induce type I collagen synthesis via the activation of the TbetaRI kinase-independent Smad pathway. |
| Databáze: | OpenAIRE |
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