Toll-Like Receptors -2, -3, -4 and -7 Expression Patterns in the Liver of a CLP-Induced Sepsis Mouse Model
| Autor: | Anastasios Machairas, Dimitrios Schizas, Theano Demesticha, Theodore Liakakos, Alkiviadis Kostakis, Demetrios Moris, Stylianos Vagios, Chrysostomos V Aravanis, Michalis Katsimpoulas, Alkistis Kapelouzou, Diamantis I. Tsilimigras |
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| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_treatment Punctures Real-Time Polymerase Chain Reaction Severity of Illness Index law.invention Andrology Sepsis Mice 03 medical and health sciences 0302 clinical medicine Liver Function Tests law Laparotomy Animals Humans Medicine Liver damage Receptor Cecum Ligation Polymerase chain reaction medicine.diagnostic_test business.industry Gene Expression Profiling Toll-Like Receptors medicine.disease Pathophysiology Mice Inbred C57BL Disease Models Animal Liver 030220 oncology & carcinogenesis Immunohistochemistry 030211 gastroenterology & hepatology Surgery business Liver function tests |
| Zdroj: | Journal of Investigative Surgery. 33:109-117 |
| ISSN: | 1521-0553 0894-1939 |
| DOI: | 10.1080/08941939.2018.1476630 |
| Popis: | Objective: To investigate the expression of toll-like receptors (TLRs) in the liver of septic mouse model. Materials and methods: For this study seventy-two C57BL/6J mice were utilized. Sepsis was induced by cecal ligation and puncture (CLP) in the mice of the three septic (S) groups (euthanized at 24 hours, 48 hours and 72 hours). Sham (laparotomy)- operated mice constituted the control (C) groups (euthanized at 24, 48 and 72 hours). Blood samples were drawn and liver tissues were extracted and examined histologically. The expression of TLRs 2, 3, 4 and 7 was assessed via immunohistochemistry (IHC) and qrt-PCR (quantitative- Polymerase Chain Reaction). Results: Liver function tests were elevated in all S-groups in contrast to their time-equivalent control groups (S24 versus C24, S48 versus C48 and S72 versus C72) (p 0.05); whereas at 72 hours only TLR 4 levels were significantly elevated in the intestine (p < 0.05). Conclusion: TLRs seem to be expressed in significant levels in the livers of septic rodents, indicating that they have a possible role in the pathophysiology of liver damage in septic conditions. |
| Databáze: | OpenAIRE |
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