Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway
| Autor: | Chao Qian Li, Qi Xiang Sun, Jian Lin Huang, Xiao Ju Chen, Yi En Yao, Huan Zeng, Wei Wei Liu, Jing Hong Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Medicine (General) Ovalbumin Physiology QH301-705.5 Jagged1 receptor medicine.medical_treatment Immunology Cell Biophysics Notch signaling pathway Ocean Engineering Biochemistry Mycobacterium Mice 03 medical and health sciences 0302 clinical medicine R5-920 Mycobacterium vaccae medicine Animals General Pharmacology Toxicology and Pharmaceutics Biology (General) Receptor Notch signaling Receptors Notch biology business.industry General Neuroscience Interleukin Cell Biology General Medicine biology.organism_classification Asthma respiratory tract diseases γδT17 030104 developmental biology Cytokine medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Signal transduction business Jagged-1 Protein Research Article Signal Transduction |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Volume: 53, Issue: 11, Article number: e9551, Published: 07 OCT 2020 Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research v.53 n.11 2020 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| Popis: | The objective of this study was to investigate the effect of Mycobacterium vaccae on Jagged 1 and gamma delta T17 (γδT17) cells in asthmatic mice. An asthma mouse model was established through immunization with ovalbumin (OVA). Gamma-secretase inhibitor (DAPT) was used to block the Notch signaling pathway. M. vaccae was used to treat asthma, and related indicators were measured. Blocking Notch signaling inhibited the production of γδT17 cells and secretion of cytokine interleukin (IL)-17, which was accompanied by a decrease in Jagged1 mRNA and protein expression in the treated asthma group compared with the untreated asthma group. Similarly, treatment with M. vaccae inhibited Jagged1 expression and γδT17 cell production, which was associated with decreased airway inflammation and reactivity. The Notch signaling pathway may play a role in the pathogenesis of asthma through the induction of Jagged1 receptor. On the other hand, the inhibitory effect of M. vaccae on Jagged1 receptor in γδT17 cells could be used for the prevention and treatment of asthma. |
| Databáze: | OpenAIRE |
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