Mechanism of action of hydrogen sulfide on cyclic AMP formation in rat retinal pigment epithelial cells
| Autor: | Madhura Kulkarni, Catherine A. Opere, Ya Fatou Njie-Mbye, Sunny E. Ohia |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
IBMX Glycine Adenylate kinase Cystathionine beta-Synthase Sodium hydrosulfide Retinal Pigment Epithelium Sulfides Cyclase Cellular and Molecular Neuroscience Cyclic nucleotide chemistry.chemical_compound KATP Channels Internal medicine medicine Cyclic AMP Animals Cyclooxygenase Inhibitors Cysteine Hydrogen Sulfide Cells Cultured Forskolin Cyclic nucleotide phosphodiesterase Dose-Response Relationship Drug Cystathionine gamma-Lyase Aminooxyacetic Acid Aminooxyacetic acid Sensory Systems Rats Enzyme Activation Ophthalmology Endocrinology chemistry Alkynes Prostaglandins |
| Zdroj: | Experimental eye research. 98 |
| ISSN: | 1096-0007 |
| Popis: | Hydrogen sulfide (H(2)S), a colorless gas with the pungent odor of rotten eggs has been reported to produce pharmacological actions in ocular and non-ocular tissues. We have evidence that H(2)S, using sodium hydrosulfide (NaHS) and sodium sulfide (Na(2)S) as donors can increase cyclic AMP (cAMP) production in neural retina. In the present study, we investigated the mechanism of action of H(2)S on cyclic nucleotide production in rat retinal pigment epithelial cells (RPE-J). Cultured RPE-J cells were incubated for 30 min in culture medium containing the cyclic nucleotide phosphodiesterase (PDE) inhibitor, IBMX (2 mM). Cells were exposed to varying concentrations of NaHS, the H(2)S substrate (L-cysteine), cyclooxygenase (COX) inhibitors or the diterpene activator of adenylate cyclase, forskolin in the presence or absence of H(2)S biosynthetic enzymes or the ATP-sensitive potassium (K(ATP)) channel antagonist, glibenclamide. Following drug-treatment at different time intervals, cell homogenates were prepared for cAMP assay using a well established methodology. In RPE-J cells, NaHS (10 nM-1 μM) produced a time-dependent increase in cAMP concentrations over basal levels which reached a maximum at 20 min. At this time point, both NaHS (1 nM-100 μM) and L-cysteine (1 nM-10 μM) produced a concentration-dependent significant (p |
| Databáze: | OpenAIRE |
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