Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome
| Autor: | Takuro Nakano, Toshikatsu Shinka, Keigo Kinoshita, Hiroki Kurahashi, Susumu Ito, Gang Chen, Emi Kiyokage, Mayumi Umeno, Kazunori Toida, Youichi Sato, Yutaka Nakahori, Hiroe Kowa |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
DNA Complementary Molecular Sequence Data Gene Expression In situ hybridization Biology Y chromosome General Biochemistry Genetics and Molecular Biology Mice Heat Shock Transcription Factors Species Specificity HSFY Expression analysis HSF medicine Animals Humans Amino Acid Sequence Heat-Shock Proteins mHSFYL Genetics Chromosomes Human Y Base Sequence Sequence Homology Amino Acid Oligospermia General Medicine Spermatids Molecular biology Recombinant Proteins spermatogenesis DNA-Binding Proteins Heat shock factor medicine.anatomical_structure Spermatogenesis Nucleus Transcription Factors |
| Zdroj: | The journal of medical investigation : JMI. 53(1-2):117-122 |
| ISSN: | 1343-1420 |
| Popis: | Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence [mHSFYL (Riken cDNA 4933413G11Rik)], which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA- or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis. |
| Databáze: | OpenAIRE |
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