The culture of primary duck endothelial cells for the study of avian influenza

Autor: Mathilde Richard, Pascale Quéré, Thijs Kuiken, Sascha Trapp, Raissa L. Davis, Geunho Choi, Kirsty R. Short
Přispěvatelé: Department of Viroscience, Erasmus University Medical Center [Rotterdam] (Erasmus MC), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, School of Chemistry and Molecular Biosciences, University of Queensland [Brisbane], Australian Infectious Diseases Research Centre, Australian Research Council DECRA (DE180100512), Department of Agriculture and Water Resources Science and Innovation Award (4-8EHOTLD), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Virology, Richard, Mathilde
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Vascular Endothelial Growth Factor A
Virologie
viruses
Endothelial cells
lcsh:QR1-502
Cell Culture Techniques
medicine.disease_cause
lcsh:Microbiology
chemistry.chemical_compound
virus influenza aviaire
Antigens
Viral
Aorta
Cells
Cultured
culture cellulaire
Methodology Article
Duck
Highly pathogenic avian influenza virus
virus diseases
Flow Cytometry
3. Good health
Endothelial stem cell
Vascular endothelial growth factor
medicine.anatomical_structure
Ducks
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Veterinary medicine and animal Health
Microbiology (medical)
animal structures
Endothelium
030106 microbiology
Bone Marrow Cells
Biology
Microbiology
Virus
03 medical and health sciences
pcr
Virology
medicine
Animals
canard
Tropism
Poultry Diseases
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Influenza A Virus
H5N1 Subtype
Influenza A virus subtype H5N1
Médecine vétérinaire et santé animal
030104 developmental biology
chemistry
Cell culture
Influenza in Birds
Bone marrow
cellule endotheliale
Zdroj: BMC Microbiology
BMC Microbiology, BioMed Central, 2018, 18 (1), pp.1-9. ⟨10.1186/s12866-018-1307-4⟩
BMC Microbiology, Vol 18, Iss 1, Pp 1-9 (2018)
BMC Microbiology, 18(1):138. BioMed Central Ltd.
BMC Microbiology 1 (18), 1-9. (2018)
ISSN: 1471-2180
Popis: Background: Endothelial cells play a major role in highly pathogenic avian influenza (HPAI) virus pathogenesis in gallinaceous poultry species (e.g. chicken, turkey and quail). Upon infection of gallinaceous poultry with HPAI viruses, endothelial cells throughout the body become rapidly infected, leading to systemic dissemination of the virus, disseminated intravascular coagulation, oedema and haemorrhaging. In contrast, the pathogenesis of HPAI viruses in most wild bird species (e.g. duck, goose and gull species) is not associated with endothelial tropism. Indeed, viral antigen is not found in the endothelial cells of most wild bird species following infection with HPAI viruses. This differential endothelial cell tropism in avian species is poorly understood, mainly due to the absence of appropriate cell culture systems. Results: Here, we describe the isolation and purification of primary duck endothelial cells from the aorta or bone marrow of Pekin duck embryos. Cells were differentiated in the presence of vascular endothelial growth factor and, if needed, enriched via fluorescent-activated cell sorting based on the uptake of acetylated low-density lipoprotein. The expression of von Willebrand factor, a key marker of endothelial cells, was confirmed by polymerase chain reaction. Monocultures of duck endothelial cells, either derived from the aorta or the bone marrow, were susceptible to infection with an H5N1 HPAI virus but to a much lesser extent than chicken endothelial cells. Conclusions: The methods described herein to isolate and purify duck endothelial cells from the aorta or bone marrow could also be applied to obtain microvascular endothelial cells from other tissues and organs, such as the lung or the intestine, and represent a valuable tool to study the pathogenesis of avian viruses.
Databáze: OpenAIRE
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