A decade of viral mutations and associated drug resistance in a population of HIV-1+ Puerto Ricans: 2002–2011

Autor: Eddy Ríos-Olivares, I. King Jordan, Lavanya Rishishwar, Maria Luisa Rogers, Lycely del C. Sepúlveda-Torres, Nawal M. Boukli, Luis A. Cubano
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
RNA viruses
Male
lcsh:Medicine
Drug resistance
medicine.disease_cause
Pathology and Laboratory Medicine
Biochemistry
Geographical locations
Database and Informatics Methods
0302 clinical medicine
Immunodeficiency Viruses
HIV Protease
Medicine and Health Sciences
030212 general & internal medicine
lcsh:Science
Genetics
Mutation
education.field_of_study
Multidisciplinary
Antimicrobials
Microbial Mutation
Drugs
Antiretrovirals
Proteases
Resistance mutation
Antivirals
3. Good health
Enzymes
Medical Microbiology
Viral Pathogens
Viruses
Female
Pathogens
Research Article
Genotype
Anti-HIV Agents
Population
Research and Analysis Methods
Microbiology
Virus
03 medical and health sciences
Acquired immunodeficiency syndrome (AIDS)
Microbial Control
Virology
Retroviruses
Drug Resistance
Viral
medicine
Humans
education
Genotyping
Microbial Pathogens
Pharmacology
Caribbean
business.industry
lcsh:R
Lentivirus
Puerto Rico
Organisms
Biology and Life Sciences
HIV
Proteins
medicine.disease
Reverse transcriptase
030104 developmental biology
Biological Databases
Mutation Databases
North America
HIV-1
Enzymology
lcsh:Q
Antimicrobial Resistance
People and places
business
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 5, p e0177452 (2017)
ISSN: 1932-6203
Popis: Puerto Rico has one of the highest rates of HIV/AIDS seen for any US state or territory, and antiretroviral therapy has been a mainstay of efforts to mitigate the HIV/AIDS public health burden on the island. We studied the evolutionary dynamics of HIV-1 mutation and antiretroviral drug resistance in Puerto Rico by monitoring the population frequency of resistance-associated mutations from 2002 to 2011. Whole blood samples from 4,475 patients were analyzed using the TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System in the Immunoretrovirus Research Laboratory at Universidad Central del Caribe. Results show that 64.0% of female and 62.9% of male patients had HIV-1 mutations that confer resistance to at least one antiretroviral medication. L63P and M184V were the dominant mutations observed for the protease (PRO) and reverse transcriptase (RT) encoding genes, respectively. Specific resistance mutations, along with their associated drug resistance profiles, can be seen to form temporal clusters that reveal a steadily changing landscape of resistance trends over time. Both women and men showed resistance mutations for an average of 4.8 drugs over the 10-year period, further underscoring the strong selective pressure exerted by antiretrovirals along with the rapid adaptive response of HIV. Nevertheless, both female and male patients showed a precipitous decrease for overall drug resistance, and for PRO mutations in particular, over the entire course of the study, with the most rapid decrease in frequency seen after 2006. The reduced HIV-1 mutation and drug resistance trends that we observed are consistent with previous reports from multi-year studies conducted around the world. Reduced resistance can be attributed to the use of more efficacious antiretroviral drug therapy, including the introduction of multi-drug combination therapies, which limited the ability of the virus to mount rapid adaptive responses to antiretroviral selection pressure.
Databáze: OpenAIRE
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