Bovine milk immunoglobulins for passive immunity to infantile rotavirus gastroenteritis
| Autor: | Josette Sidoti, P Bachmann, C Mietens, I Walther, Helmut Hilpert, Harald Brüssow |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Rotavirus
Microbiology (medical) Serotype medicine.medical_treatment Immunoglobulins Passive immunity Biology Antibodies Viral medicine.disease_cause Rotavirus Infections Neutralization Cell Line Microbiology fluids and secretions Neutralization Tests Pregnancy Lactation medicine Animals Humans Vaccination Immunization Passive Antibody titer food and beverages Virology Gastroenteritis Milk medicine.anatomical_structure biology.protein Cattle Female Antibody Research Article |
| Zdroj: | Journal of Clinical Microbiology. 25:982-986 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.25.6.982-986.1987 |
| Popis: | Pregnant cows were successfully hyperimmunized with all four human rotavirus serotypes, resulting in a 100-fold increase in neutralizing milk antibody titers over those of controls. Milk antibodies were isolated batchwise from 1,000 kg of pooled milk for the first 10 lactation days, yielding 10 kg of freeze-dried milk immunoglobulin concentrate consisting of 50% bovine milk immunoglobulins. Milk immunoglobulin concentrate showed neutralizing activities against all four human rotavirus serotypes that were 100 times higher than those in pooled human milk samples and 10 times higher than those in a commercial pooled immunoglobulin preparation from pooled human blood serum. In vitro neutralization tests showed that milk immunoglobulin concentrate had powerful antiviral activity, even against very high doses of infectious rotaviruses. Because the technology of the milk immunoglobulin concentrate ensures that it is innocuous and can be used for oral application, it is proposed that milk immunoglobulin concentrate be used to induce passive immunity to infantile rotavirus gastroenteritis. |
| Databáze: | OpenAIRE |
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