Advanced antifouling zwitterionic layer based impedimetric HER2 biosensing in human serum: Glycoprofiling as a novel approach for breast cancer diagnostics
| Autor: | Darina Velicova, Alena Holazova, Alica Vikartovská, Daniel Hasko, Pavol Farkaš, Tomas Bertok, Anita Andicsova Eckstein, Vladimir Bella, Lenka Lorencova, Peter Kasak, Jaroslav Mosnáček, Erika Chocholova, Jan Tkac |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Glycan
Glycans Glycoprofiling Photoimmobilization 02 engineering and technology macromolecular substances 010402 general chemistry 01 natural sciences Biofouling Breast cancer HER2 Materials Chemistry Zwitterion Electrical and Electronic Engineering skin and connective tissue diseases Instrumentation Diagnostics Chromatography biology Chemistry Metals and Alloys technology industry and agriculture Lectin Biomarker 021001 nanoscience & nanotechnology Condensed Matter Physics Serum samples 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Self-healing hydrogels biology.protein 0210 nano-technology Biosensor Layer (electronics) Protein adsorption |
| Popis: | There are two key novel components presented here behind identification of potential novel breast cancer (BCa) diagnostic approach: 1. application of photoimmobilizable zwitterionic hydrogels resisting non-specific protein adsorption for preparation of the biosensor interfaces and 2. integration of lectins (carbohydrate recognizing proteins) within biosensors to evaluate changes in the glycan profile of HER2 protein on the molecular level. A disposable, electrochemical biosensor based on screen printed carbon electrodes (SPCEs) with a deposited hydrogel layer was applied for covalent attachment of antibodies for a specific interaction with HER2. In the subsequent step, HER2 molecules were in situ glycoprofiled using lectins. The impedimetric immunosensor was able to detect HER2 down to 5 pg mL?1 (?77 fM) with a minimal non-specific protein adsorption. The biosensor was then combined with lectins to glycoprofile HER2 in two serum samples (one from a healthy, high BCa risk woman and the other from a woman with a 2nd stage BCa). The results obtained by the glycoprofiling via the impedimetric biosensor were successfully verified by independent lectin-based enzyme-linked immunosorbent assays (ELISA). To our best knowledge it is a very first biosensor applying zwitterionic polymeric hydrogel-modified interface for glycoprofiling of a cancer biomarker. Financial support received from the Slovak Scientific Grant Agency VEGA 2/0162/14 and Slovak Research and Development Agency APVV 17-0300 is acknowledged. The research received funding from the European Research Council (No. 311532 ). This publication is the result of the project implementation: Centre for materials, layers and systems for applications and chemical processes under extreme conditions Stage I, ITMS No.: 26240120007, supported by the ERDF . Appendix A Scopus |
| Databáze: | OpenAIRE |
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