Effect of intermittent and continuous exposure to electromagnetic fields on cultured hippocampal cells
| Autor: | D. Delapierre, André Boland, Danielle Mossay, Albert Dresse, Vincent Seutin |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Nitroprusside
medicine.medical_specialty Programmed cell death Basal rate animal structures Cell Survival Physiology Cell Biophysics Hippocampal formation Biology medicine.disease_cause Hippocampus Nitric oxide Toxicology chemistry.chemical_compound Electromagnetic Fields Internal medicine Lactate dehydrogenase medicine Humans Nitric Oxide Donors Radiology Nuclear Medicine and imaging Ferrous Compounds Cells Cultured Cell Death General Medicine Oxidative Stress medicine.anatomical_structure Endocrinology chemistry Sodium nitroprusside Oxidative stress medicine.drug |
| Zdroj: | Bioelectromagnetics. 23:97-105 |
| ISSN: | 1521-186X 0197-8462 |
| DOI: | 10.1002/bem.102 |
| Popis: | This study was designed to assess the effect of 50 Hz electromagnetic fields (EMFs) on hippocampal cell cultures in the presence or absence of either sodium nitroprusside (SNP, a NO donor) or Fe2+ induced oxidative stress. One week old cultured rat hippocampal cells were exposed to either intermittent EMFs (IEMFs, 50 Hz, 0–5 mT, 1 min ON/OFF cycles, repeated 10 times every 2 h, 6 times/day during 48 h) or continuous EMFs (CEMFs, 50 Hz, 0–5 mT for 48 h). In a second set of experiments, the effect on such EMFs applied in combination with oxidative stress induced by 0.5 μM Fe2+ or SNP was estimated. At the end of both sets of experiments, cell mortality was assessed by lactate dehydrogenase measurements (LDH). Neither type of exposure to EMFs was observed to modify the basal rate of cell mortality. The exposure to CEMFs in presence of either NO or Fe2+ did not induce any significant increase in cell death. However, when cells were exposed to EMFs in the presence of NO, we observed a significant increase in cell death of 11 and 23% (P |
| Databáze: | OpenAIRE |
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