Fluorescent labelling of nascent RNA reveals nuclear transcription domains throughout plant cell nuclei
| Autor: | K.R. Straatman, J.H.N. Schel, W. Schul, C.M. Trompetter |
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| Přispěvatelé: | Synthetic Systems Biology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Laboratorium voor Plantencelbiologie
Nucleolus Confocal scanning laser microscopy Plant Science Biology Transcription (biology) Labelling Isolated nuclei medicine Transcription sites Rna processing Brassica napus food and beverages RNA Cell Biology General Medicine Plant cell Molecular biology Immunolabelling Cell biology Laboratory of Plant Cell Biology Fluorescent labelling medicine.anatomical_structure 5-Bromouridine 5'-triphosphate incorporation EPS Nucleus |
| Zdroj: | Protoplasma, 192, 145-149 Scopus-Elsevier Protoplasma, 192, 145-149. Springer Wien Protoplasma 192 (1996) |
| ISSN: | 0033-183X |
| DOI: | 10.1007/bf01273886 |
| Popis: | Transcription and RNA processing are main functions of the nucleus. These processes are found localized in specific nuclear domains. We have investigated the presence of nuclear transcription domains in plants by incorporating 5-bromouridine 5′-triphosphate in nascent RNA in isolated nuclei ofBrassica napus. Bromo-uridine labelled RNA was visualized by a FITC-labelled biotin-avidin system in combination with confocal laser scanning microscopy. Labelled domains were found throughout the nucleus, in some cases including the nucleolus. This shows that the distribution of transcription sites in plant nuclei is similar to that of mammalian nuclei and that the same labelling procedures can be used. |
| Databáze: | OpenAIRE |
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