Regulation of ZO-1 on β-catenin mediates sulforaphane suppressed colorectal cancer stem cell properties in colorectal cancer

Autor:	Yue, Chen, LvYuwei, Tang, Xinrong, Ye, Yimeng, Chen, Enfang, Shan, Hongyu, Han, Caiyun, Zhong
Rok vydání:	2022
Předmět:	Gene Expression Regulation Neoplastic Cell Line Tumor Neoplastic Stem Cells Zonula Occludens-1 Protein Humans General Medicine Colorectal Neoplasms Wnt Signaling Pathway beta Catenin Cell Proliferation Food Science
Zdroj:	Food & Function. 13:12363-12370
ISSN:	2042-650X 2042-6496
DOI:	10.1039/d2fo02932d
Popis:	Cancer stem cells (CSCs) function as the driving force of cancer initiation and progression. Wnt/β-catenin is the core pluripotency pathway in CSCs, while its crucial regulator has not been fully elucidated yet. Here, we evaluated the role of ZO-1, a component of the tight junction protein complex, in colorectal CSCs, and found ZO-1 downregulation in both colorectal cancer cells and spheres. Over-expression of ZO-1 can inhibit the sphere-forming capacity and CSC marker expression in spheres. Immunofluorescence staining and co-immunoprecipitation analysis further revealed the interaction between ZO-1 and β-catenin and the repressed role of ZO-1 in β-catenin nuclear accumulation. Using
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbb6f6f4adbb38a065ea54eb8e525c88 https://doi.org/10.1039/d2fo02932d Zobrazit plný text záznamu