Size and distribution of polyadenylic acid sequences in Drosophila polytene DNA and RNA
| Autor: | Montserrat Pages, M.Luisa García, Carlos Alonso |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
chemistry.chemical_classification
Poly U Polytene chromosome biology RNA Chromosome Nucleic Acid Hybridization DNA In Vitro Techniques biology.organism_classification Biochemistry Genetics and Molecular Biology (miscellaneous) Genome Molecular biology chemistry.chemical_compound Ribonucleases chemistry Drosophila hydei biology.protein Animals Nucleotide Drosophila Ribonuclease Poly A |
| Zdroj: | Biochimica et biophysica acta. 479(3) |
| ISSN: | 0006-3002 |
| Popis: | [3H]Poly(U) hybridizes very rapidly to polytene DNA from Drosophila hydei. When hybridization is performed at 30 degrees C in 2 X SSC to a large excess of DNA, 95% of the poly(U) becomes ribonuclease resistant. Also, complementary RNA transcribed in vitro from polytene DNA hybridizes to poly(U). 023--0.25% of the DNA is composed of (dA)-rich sequences and 0.23--0.31% of cRNA hybridizes to [3H]poly(U). The length of the (dA)-rich sequences on the DNA and cRNA is 40 nucleotides. The Tm values of these hybrids formed between DNA or cRNA-poly(U) is 45 degrees C. The poly(A) fragments from cytoplasmic RNA ranged from 80 to 170 nucleotides in lenght, and migrated in polyacrilamide gels as a broad peak. The average sizes of the poly(A) fragments from the poly(A)-containing RNA transcribed by nuclei isolated from salivary glands in vivo or in vitro were 40, 70, 170 and 70 nucleotides, respectively. Hybridization in situ of [3H]-poly(U) to chromosome squashes indicated that the (dA)-rich sequences are randomly distributed over the whole genome. |
| Databáze: | OpenAIRE |
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