Microsomal membrane proteome of low grade diffuse astrocytomas: Differentially expressed proteins and candidate surveillance biomarkers
| Autor: | Praveen Ankathi, Akhila Lakshmikantha, Rakesh Kumar Sharma, Challa Sundaram, Ravindra Varma Polisetty, Kiran Mariswamappa, Durairaj Renu, Bhadravathi Marigowda Shivakumar, Ravi Sirdeshmukh, Poonam Gautam, Megha S Uppin, Harsha Gowda, Manoj Kumar Gupta, Aniruddh Kumar Purohit |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Proteome Carcinogenesis Astrocytoma Biology Article 03 medical and health sciences Diffuse Astrocytoma Microsomes Biomarkers Tumor medicine Humans Amino Acid Sequence Peptide sequence Multidisciplinary Brain Neoplasms Gene Expression Profiling TOR Serine-Threonine Kinases Computational Biology Molecular Sequence Annotation Intracellular Membranes medicine.disease Fold change Neoplasm Proteins 3. Good health Gene Expression Regulation Neoplastic Gene expression profiling 030104 developmental biology Membrane protein Case-Control Studies Cancer research Female Neoplasm Grading Neoplasm Recurrence Local Signal Transduction Anaplastic astrocytoma |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep26882 |
| Popis: | Diffuse astrocytoma (DA; WHO grade II) is a low-grade, primary brain neoplasm with high potential of recurrence as higher grade malignant form. We have analyzed differentially expressed membrane proteins from these tumors, using high-resolution mass spectrometry. A total of 2803 proteins were identified, 340 of them differentially expressed with minimum of 2 fold change and based on ≥2 unique peptides. Bioinformatics analysis of this dataset also revealed important molecular networks and pathways relevant to tumorigenesis, mTOR signaling pathway being a major pathway identified. Comparison of 340 differentially expressed proteins with the transcript data from Grade II diffuse astrocytomas reported earlier, revealed about 190 of the proteins correlate in their trends in expression. Considering progressive and recurrent nature of these tumors, we have mapped the differentially expressed proteins for their secretory potential, integrated the resulting list with similar list of proteins from anaplastic astrocytoma (WHO Grade III) tumors and provide a panel of proteins along with their proteotypic peptides, as a resource that would be useful for investigation as circulatory plasma markers for post-treatment surveillance of DA patients. |
| Databáze: | OpenAIRE |
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