Real-time measurement of small molecules directly in awake, ambulatory animals
| Autor: | Kyle L. Ploense, Kevin W. Plaxco, Philip A. Vieira, Tod E. Kippin, Netzahualcóyotl Arroyo-Currás, Jacob Somerson |
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| Rok vydání: | 2017 |
| Předmět: |
Male
precision medicine E-DNA Bioengineering 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Aptamers Rats Sprague-Dawley Small Molecule Libraries Pharmacokinetics Limit of Detection square-wave voltammetry Medicine Animals Humans Multidisciplinary business.industry aptamer Aptamers Nucleotide 021001 nanoscience & nanotechnology Small molecule 0104 chemical sciences Rats in vivo Good Health and Well Being Pharmaceutical Preparations 5.1 Pharmaceuticals Drug delivery Ambulatory Physical Sciences Cattle Personalized medicine Sprague-Dawley Generic health relevance Development of treatments and therapeutic interventions 0210 nano-technology business Nucleotide Biomedical engineering Biotechnology |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 114, iss 4 |
| Popis: | The development of a technology capable of tracking the levels of drugs, metabolites, and biomarkers in the body continuously and in real time would advance our understanding of health and our ability to detect and treat disease. It would, for example, enable therapies guided by high-resolution, patient-specific pharmacokinetics (including feedback-controlled drug delivery), opening new dimensions in personalized medicine. In response, we demonstrate here the ability of electrochemical aptamer-based (E-AB) sensors to support continuous, real-time, multihour measurements when emplaced directly in the circulatory systems of living animals. Specifically, we have used E-AB sensors to perform the multihour, real-time measurement of four drugs in the bloodstream of even awake, ambulatory rats, achieving precise molecular measurements at clinically relevant detection limits and high (3 s) temporal resolution, attributes suggesting that the approach could provide an important window into the study of physiology and pharmacokinetics. |
| Databáze: | OpenAIRE |
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