MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-β-Treated Endometrial Stromal Cells via the MAPK and Wnt/β-Catenin Signaling Pathways
| Autor: | Jingwen Ma, Jun Wei, Liwei Yuan, Linlin Wang, Yani Huang, Zhijuan Yang, Shiyun Zhao, Dan Liu |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Cancer Research Epithelial-Mesenchymal Transition Stromal cell Apoptosis Pathology and Forensic Medicine MAPK1 Extracellular matrix 03 medical and health sciences 0302 clinical medicine miRNA-543 Cell Movement Endometrial Stromal Tumors Transforming Growth Factor beta endometrial stromal cell Biomarkers Tumor Tumor Cells Cultured Humans molecular biology Epithelial–mesenchymal transition Protein kinase A beta Catenin Cell Proliferation Original Research Endometrial Stromal Cell Chemistry Wnt signaling pathway General Medicine Endometrial Neoplasms Cell biology Gene Expression Regulation Neoplastic Wnt Proteins MicroRNAs Society Journal Archive 030104 developmental biology Oncology 030220 oncology & carcinogenesis embryonic structures Female Mitogen-Activated Protein Kinases Signal transduction wnt/β-catenin |
| Zdroj: | Pathology and Oncology Research |
| ISSN: | 1532-2807 |
| Popis: | Intrauterine adhesion (IUA) is one of the most prevalent reproductive system diseases in females. MicroRNAs (miRNAs) are reported to be master regulators in a variety of diseases, including IUA, but the role of microRNA-543 (miR-543) in IUA remains to be elucidated. In this study, we observed that miR-543 was downregulated in transforming growth factor-beta (TGF-β)-treated endometrial stromal cells (ESCs). Functionally, we observed that miR-543 suppressed the migration, epithelial-to-mesenchymal transition (EMT), and inhibited expression of extracellular matrix (ECM) proteins in TGF-β-treated ESCs. Mechanistically, MAPK1 is targeted by miR-543 after prediction and screening. A luciferase reporter assay demonstrated that miR-543 complementarily binds with the 3′ untranslated region of mitogen-activated protein kinase 1 (MAPK1), and western blot analysis indicated that miR-543 negatively regulates MAPK1 protein levels. In addition, results from rescue assays showed that miR-543 inhibits the migration and EMT of TGF-β-treated ESCs by targeting MAPK1. In addition, we observed that miR-543 inactivates the Wnt/β-catenin signaling pathway through inhibiting the phosphorylation of MAPK1 and β-catenin. Finally, we confirmed that miR-543 represses migration, EMT and inhibits levels of ECM proteins in TGF-β-treated ESCs by targeting the Wnt/β-catenin signaling pathway. Our results demonstrated that miR-543 suppresses migration and EMT of TGF-β-treated ESCs by targeting the MAPK and Wnt/β-catenin pathways. |
| Databáze: | OpenAIRE |
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