Allele- and parent-of-origin-specific effects on expression of the KCNJ11 gene: A candidate for meat tenderness in cattle
| Autor: | Luciana Correia de Almeida Regitano, M. M. de Souza, M. I. P. Rocha, S. C. M. Niciura, Flavia A. Bressani, W. Malagó, G. Gasparin, Wellison Jarles da Silva Diniz, W. Barioni Junior, P. S. N. de Oliveira, Polyana C. Tizioto, Luiz Lehmann Coutinho, Andressa Oliveira de Lima, Maurício A. Mudadu, Adriana Mércia Guaratini Ibelli |
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| Přispěvatelé: | M.M. de Souza, UFSCAR, SIMONE CRISTINA MEO NICIURA, CPPSE, P.C. Tizioto, FAPESP, ADRIANA MERCIA GUARATINI IBELLI, CNPSA, G. Gasparin, USP-ESALQ, M. I. P. Rocha, UFSCAR, FLAVIA ALINE BRESSANI DONATONI, CPPSE, WILSON MALAGO JUNIOR, CPPSE, W. J. S. Diniz, UFSCAR, Priscila Silva Neubern de Oliveira, FAPESP, A. O. Lima, UFSCAR, MAURICIO DE ALVARENGA MUDADU, CNPTIA, WALDOMIRO BARIONI JUNIOR, CPPSE, L. L. Coutinho, USP-ESALQ, LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
| Rok vydání: | 2016 |
| Předmět: |
Genetic Markers
Male 0301 basic medicine medicine.medical_specialty Candidate gene Meat POTÁSSIO Genotype Inheritance Patterns Maciez da carne Gene Expression Single-nucleotide polymorphism Quantitative trait locus Biology Polymorphism Single Nucleotide 03 medical and health sciences Meat tenderness Quantitative Trait Heritable Molecular genetics Genetics medicine Animals Potassium Channels Inwardly Rectifying Allele Muscle Skeletal Molecular Biology Gene Alleles Nelore Gado Nelore General Medicine Expressão alélica diferencial Phenotype 030104 developmental biology Cattle Female |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| Popis: | In contrast to the Mendelian inheritance model, parental alleles can contribute unequally to gene expression, which may result in phenotypic variance among individuals and bias in the predicted additive effect of molecular markers associated with production traits. Given the need to understand the effects of allelic variation and parent-of-origin effects on the expression of genes with a commercial interest in cattle, we analyzed the expression of KCNJ11 (potassium inwardly rectifying channel, subfamily J, member 11), which was previously described as a functional candidate gene for meat tenderness. Allele-specific and parent-of-origin-dependent expression of this gene were assessed in bovine muscle using the rs379610823 single nucleotide polymorphism as a reference. Biallelic expression was observed; however, the T allele was expressed at significantly higher levels than the C allele. Furthermore, increased expression of KCNJ11 was found in animals harboring the maternal T allele. This study is the first to describe the differential allelic expression of bovine KCNJ11. Our findings are important for understanding the mechanisms that underlie the pattern of KCNJ11 expression and its potential impact on the phenotypic variation of meat tenderness in Nelore beef cattle. This reinforces the need for further investigation of allelic- and parent-of-origin expression deviation in genetic markers eligible for the selection of target traits. |
| Databáze: | OpenAIRE |
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