A genotoxicity study on Talitrus saltator (Crustacea, Amphipoda) exposed to lead and benzo(a)pyrene
Autor: | Claudio Chimenti, G. Di Donato, Domenico Davolos, E. De Matthaeis |
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Rok vydání: | 2020 |
Předmět: |
Pollution
Amphipoda biology Chemistry media_common.quotation_subject polycyclic aromatic hydrocarbons comet assay DNA damage hemocytes heavy metals Talitrus saltator Heavy metals biology.organism_classification medicine.disease_cause Crustacean chemistry.chemical_compound Benzo(a)pyrene Environmental chemistry lcsh:Zoology medicine Animal Science and Zoology dna damage lcsh:QL1-991 Genotoxicity media_common |
Zdroj: | The European Zoological Journal, Vol 87, Iss 1, Pp 235-241 (2020) |
ISSN: | 2475-0263 |
DOI: | 10.1080/24750263.2020.1751887 |
Popis: | Sandy beaches are ecosystems often subjected to a variety of pollution sources, including heavy metals and polycyclic aromatic hydrocarbons, largely due to improper human activities. The sandhopper Talitrus saltator s. l. (Montagu, 1808) typically lives on supralittoral shores where it plays an important ecological role. The purpose of this study was to investigate DNA damage levels on hemocytes of T. saltator by means of the Comet assay. Firstly, we tested the sensitivity and reproducibility of the Comet assay on hemocytes of T. saltator after in vitro exposure (1 h) to the genotoxicant methyl methanesulfonate (MMS). The data demonstrated a DNA damage (defined as % DNA tail) related to MMS exposure in a concentration-dependent manner. Next, we carried out the assay on hemocytes of T. saltator that were exposed in vivo (24 h and 7 days) to (a) lead (Pb), (b) benzo(a)pyrene (B(a)P), (c) mixtures of them. Our data on exposure to Pb or B(a)P showed DNA damage on the hemocytes of T. saltator in a concentration-dependent manner, but apparently with a non-synergistic effect of exposure to their mixtures. Our results provide a background for further studies in order to verify the applicability of Comet assay on T. saltator for assessing genotoxicity levels in sandy beaches subjected to pollution. |
Databáze: | OpenAIRE |
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