Random amplified polymorphic DNA analysis of DNA extracted from Trichuris trichiura (Linnaeus, 1771) eggs and its prospective application to paleoparasitological studies
| Autor: | Erika Verissimo Villela, Jorge Antonio Santos Correia, Antonio Nascimento Duarte, Alexandre Ribeiro Bello, Elaine Machado Martinez, Luiz Fernando Ferreira |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Genetic Markers
Microbiology (medical) lcsh:Arctic medicine. Tropical medicine Paleoparasitology lcsh:RC955-962 Base pair Molecular Sequence Data lcsh:QR1-502 Biology lcsh:Microbiology DNA sequencing chemistry.chemical_compound random amplified polymorphic DNA analysis Animals Humans Trichiura Trichuris trichiura Ovum Electrophoresis Agar Gel Genetics Base Sequence DNA Helminth biology.organism_classification Molecular biology Random Amplified Polymorphic DNA Technique Trichuris chemistry Genetic marker Agarose paleoparasitology DNA |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 98 Supplement 1, Pages: 59-62, Published: JAN 2003 ResearcherID Memórias do Instituto Oswaldo Cruz., Vol 98, Pp 59-62 (2003) |
| Popis: | Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing. T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches. |
| Databáze: | OpenAIRE |
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