Single-component supported lipid bilayers probed using broadband nonlinear optics
| Autor: | Hong-fei Wang, Laura L. Olenick, Hilary M. Chase, Franz M. Geiger, Yun Zhang, Li Fu, Merve Doğangün, Stephanie R. Walter, Alicia C. McGeachy |
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| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Chemistry Infrared Bilayer Analytical chemistry General Physics and Astronomy Nonlinear optics 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences chemistry.chemical_compound Physical and Theoretical Chemistry 0210 nano-technology Spectroscopy Lipid bilayer POPC Alkyl Methyl group |
| Zdroj: | Physical chemistry chemical physics : PCCP. 20(5) |
| ISSN: | 1463-9084 |
| Popis: | Broadband SFG spectroscopy is shown to offer considerable advantages over scanning systems in terms of signal-to-noise ratios when probing well-formed single-component supported lipid bilayers formed from zwitterionic lipids with PC headgroups. The SFG spectra obtained from bilayers formed from DOPC, POPC, DLPC, DMPC, DPPC and DSPC show a common peak at ∼2980 cm−1, which is subject to interference between the C–H and the O–H stretches from the aqueous phase, while membranes having transition temperatures above the laboratory temperature produce SFG spectra with at least two additional peaks, one at ∼2920 cm−1 and another at ∼2880 cm−1. The results validate spectroscopic and structural data from SFG experiments utilizing asymmetric bilayers in which one leaflet differs from the other in the extent of deuteration. Differences in H2O–D2O exchange experiments reveal that the lineshapes of the broadband SFG spectra are significantly influenced by interference from OH oscillators in the aqueous phase, even when those oscillators are not probed by the incident infrared light in our broadband setup. In the absence of spectral interference from the OH stretches of the solvent, the alkyl chain terminal methyl group of the bilayer is found to be tilted at an angle of 15° to 35° from the surface normal. |
| Databáze: | OpenAIRE |
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