Characterization and osteogenic effects of mesenchymal stem cells on microbeads composed of hydroxyapatite nanoparticles/reconstituted collagen
| Autor: | Long Bin Jeng, Sophia Chia-Ning Chang, Yi Lung Lu, Jyh-Ping Chen, Yng Jiin Wang, Tsung Min Lin, Yi Ling Chen, Hui Ying Chung |
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| Rok vydání: | 2009 |
| Předmět: |
Artificial bone
Materials science Biomedical Engineering Matrix (biology) Flow cytometry law.invention Biomaterials Focal adhesion Osteogenesis Confocal microscopy law medicine Animals CD11a Antigen Microscopy Confocal medicine.diagnostic_test Mesenchymal stem cell Metals and Alloys Mesenchymal Stem Cells Flow Cytometry Actins Microspheres Staining Durapatite Hyaluronan Receptors Microscopy Electron Scanning Ceramics and Composites Biophysics Nanoparticles Thy-1 Antigens Alkaline phosphatase Cattle Collagen Rabbits Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. :886-893 |
| ISSN: | 1552-4965 1549-3296 |
| Popis: | Natural bone is comprised of nanosized blade-like crystals of hydroxyapatite grown in close contact with collagen (Col) fibers. Characteristics of artificial bone tissue differ considerably with those of natural ones, mainly from the unusual self-organizing interaction between the apatite crystals and the proteic components. Nanoparticle spheres of hydroxyapatite (n-HA), dispersed in reconstituted fibrous Col, were prepared in three weight ratios of 75:25, 65:35, and 50:50 (n-HA:Col). Bone marrow mesenchymal stem cells (MSCs) from rabbits were seeded and cultured on the n-HA/Col microbeads and characterized. n-HA were evenly distributed throughout the Col matrix and aggregated to microbeads as determined by scanning electron microscopy. Electron and confocal microscopy showed that the MSCs spread and attached to microbeads via focal adhesions, while staining for F-actin and DNA revealed the presence of stress fibers. The phenotype of the MSCs in the flow cytometry was identified as CD11a-, CD44+, and CD90.1+. The optimal weight ratio is 65:35 for the normalized alkaline phosphatase activities. The transduced MSCs, engineered by replication-defective adenovirus to express the BMP-2 gene, demonstrated synergic osteogenic effects in the microbeads. MSCs are capable of proliferating and differentiating in appropriate combinations of n-HA/Col. Thus it is a promising composite for future clinical applications. |
| Databáze: | OpenAIRE |
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