UHPLC-MS/MS method for the simultaneous quantitation of five anthraquinones and gallic acid in rat plasma after oral administration of prepared rhubarb decoction and its application to a pharmacokinetic study in normal and acute blood stasis rats
| Autor: | Ke Pei, Xiao Liu, Kun Ming Qin, Hui Zhu, Bao Chang Cai, Tingting Zhu, Xiaoli Wang |
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| Rok vydání: | 2017 |
| Předmět: |
Formic acid
Administration Oral Filtration and Separation Decoction Anthraquinones Tandem mass spectrometry 030226 pharmacology & pharmacy Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Tandem Mass Spectrometry Gallic Acid Animals Gallic acid Rheum Chromatography High Pressure Liquid Chromatography Extraction (chemistry) Rats chemistry 030220 oncology & carcinogenesis Emodin Drugs Chinese Herbal |
| Zdroj: | Journal of separation science. 40(11) |
| ISSN: | 1615-9314 |
| Popis: | Prepared rhubarb, as one of the main processed products of rhubarb, has a good effect on promoting blood circulation. In this paper we describe a rapid, sensitive, and selective ultra-fast liquid chromatography with tandem mass spectrometry method for simultaneous quantification of five anthraquinones (rhein, aloe-emodin, chrysophanol, emodin, and physcion) and gallic acid in plasma. Chromatographic separation was performed on an Extend C18 column at the temperature of 30°C using a mobile phase that consisted of 0.1% aqueous formic acid and acetonitrile. Satisfactory linearity, precision, accuracy, extraction recovery, and matrix effect have been achieved. Then, the validated method was successfully applied to a comparative pharmacokinetic study. The results might be helpful for guiding clinical application of prepared rhubarb in the future. |
| Databáze: | OpenAIRE |
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