The Effect of Gene Gun-delivered pGM-CSF on the Immunopathology of the Vaccinated Skin
| Autor: | Katie Matthews, Robert G. Dalziel, Anton Gossner, J Hopkins, S. M. Rhind |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Immunology Biology Gene gun Immune system Adjuvants Immunologic Immunopathology medicine Animals Gene Skin Sheep Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Granulocyte-Macrophage Colony-Stimulating Factor General Medicine Biolistics medicine.disease Immunohistochemistry Cytokines Female Histopathology Adjuvant Infiltration (medical) |
| Zdroj: | Scandinavian Journal of Immunology. 65:298-307 |
| ISSN: | 1365-3083 0300-9475 |
| DOI: | 10.1111/j.1365-3083.2007.01902.x |
| Popis: | The aim of this study was to investigate the skin immunopathology of gene gun-delivered plasmid-encoded granulocyte-macrophage colony-stimulating factor (pGM-CSF) and hence explore the possible mechanisms of its adjuvant activity. Using sheep as the experimental model, expressible pGM-CSF was administered to the epidermis and the dermal/epidermal junction and its effects on the skin were assessed by histopathology, immunohistology and quantitative RT-PCR for a range of pro-inflammatory and immune response-polarizing cytokines. Both functional and non-functional plasmids caused an acute inflammatory response with the infiltration of neutrophils and micro-abscess formation; however, the response to pGM-CSF was more severe and was also associated with the accumulation of eosinophils, immature (CD1b(-)/CD172a(-)) dendritic cells and B cells. In terms of cytokine expression, an early TNF-alpha response was stimulated by gene gun delivery of plasmid-associated gold beads, which coincided with an immediate infiltration of neutrophils. However, only pGM-CSF triggered the short-lived expression of GM-CSF (peaking at 4 h) and significant long-term increases in both TNF-alpha and IL-1beta. pGM-CSF did not affect the expression of the immune response-polarizing cytokines, IL-10 and IL-12. |
| Databáze: | OpenAIRE |
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