Improving therapeutic efficacy of voriconazole against fungal keratitis: Thermo-sensitive in situ gels as ophthalmic drug carriers
| Autor: | Vildan Yozgatlı, Ayşegül Yoltaş, Panoraia I. Siafaka, Mehmet Evren Okur, Neslihan Üstündağ Okur |
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| Přispěvatelé: | Okur, Neslihan Ustundag Univ Hlth Sci, Fac Pharm, Dept Pharmaceut Technol, TR-34668 Istanbul, Turkey, Yozgatli, Vildan, Siafaka, Panoraia, I Istanbul Medipol Univ, Sch Pharm, Dept Pharmaceut Technol, TR-34810 Istanbul, Turkey, Okur, Mehmet Evren Univ Hlth Sci, Fac Pharm, Dept Pharmacol, TR-34668 Istanbul, Turkey, Yoltas, Aysegul Ege Univ, Fac Sci, Dept Biol, Fundamental & Ind Microbiol Div, TR-35100 Izmir, Turkey, Siafaka, Panoraia, I Aristotle Univ Thessaloniki, Dept Chem, Thessaloniki 54124, Greece |
| Rok vydání: | 2019 |
| Předmět: |
Ex Vivo
Ocular Irritation Test Pharmaceutical Science Rabbit 02 engineering and technology Pharmacology medicine.disease_cause Microbiological Study 030226 pharmacology & pharmacy 03 medical and health sciences 0302 clinical medicine Thermo-Sensitive in Situ Gel In vivo Cornea medicine Fungal keratitis Voriconazole Chemistry Ocular Drug Delivery Poloxamer 021001 nanoscience & nanotechnology medicine.disease Bioavailability medicine.anatomical_structure Poloxamer 407 Irritation 0210 nano-technology medicine.drug |
| Zdroj: | Journal of Drug Delivery Science and Technology. 49:323-333 |
| ISSN: | 1773-2247 |
| DOI: | 10.1016/j.jddst.2018.12.005 |
| Popis: | WOS: 000457344000037 The aim of this research was to evaluate the potential use of in situ gel formulations for voriconazole ocular delivery as fungal keratitis treatment. The in situ gelling system was applied to increase residence time and the bioavailability of voriconazole in the ocular mucosa. Temperature-triggered in situ gel formulations were prepared by cold method. Poloxamer 188, poloxamer 407 and carboxymethylcellulose were used for the preparation of thermosensitive in situ ocular gel. Voriconazole concentration in formulations was 0.1% (w/w). The prepared gels were evaluated for clarity, sol-gel transition temperature, gelling capacity, pH, viscosity, FT-IR and drug content. The gelation temperatures of all the formulations were within the range of 29-34 degrees C. All formulations exhibited fairly uniform drug content. Furthermore, sterility, antifungal activity, stability, in vitro drug release, ex vivo permeation, and penetration and in vivo study of these formulations were also examined. Drug release results indicated that all formulations showed sustained release properties. Irritation studies showed that no ocular damage or clinically abnormal signs were observed in the cornea, conjunctiva or iris upon administration of the formulation. In conclusion, voriconazole loaded in situ gels could be offered as a promising strategy as ocular carriers for the treatment of fungal keratitis. |
| Databáze: | OpenAIRE |
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