Dynamique de la Chromatine , développement des plantes, ingéniérie épigénomique
| Autor: | François Roudier, Annick Dubois |
|---|---|
| Přispěvatelé: | Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), ANR-18-CE12-0014,ChromAuxi,Décodage de la réponse auxine à l'interface ARFs-chromatine(2018) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
QH301-705.5 Computer science [SDV]Life Sciences [q-bio] Health Toxicology and Mutagenesis plant Computational biology Review 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry 03 medical and health sciences Genome regulation Genetics Epigenome editing CRISPR Cellular dynamics Biology (General) epigenome engineering 030304 developmental biology 0303 health sciences CRISPR-dCas9 Epigenome Chromatin CRISPR-dC9as9 epigenome editing Medicine chromatin Transcription (software) Function (biology) 010606 plant biology & botany |
| Zdroj: | Epigenomes Epigenomes, MDPI, 2021, 5 (3), pp.17. ⟨10.3390/epigenomes5030017⟩ Epigenomes, Vol 5, Iss 17, p 17 (2021) Epigenomes, 2021, 5 (3), pp.17. ⟨10.3390/epigenomes5030017⟩ |
| ISSN: | 2075-4655 |
| Popis: | International audience; CRISPR-based epigenome editing uses dCas9 as a platform to recruit transcription or chromatin regulators at chosen loci. Despite recent and ongoing advances, the full potential of these approaches to studying chromatin functions in vivo remains challenging to exploit. In this review we discuss how recent progress in plants and animals provides new routes to investigate the function of chromatin regulators and address the complexity of associated regulations that are often interconnected. While efficient transcriptional engineering methodologies have been developed and can be used as tools to alter the chromatin state of a locus, examples of direct manipulation of chromatin regulators remain scarce in plants. These reports also reveal pitfalls and limitations of epigenome engineering approaches that are nevertheless informative as they are often associated with locus- and context-dependent features, which include DNA accessibility, initial chromatin and transcriptional state or cellular dynamics. Strategies implemented in different organisms to overcome and even take advantage of these limitations are highlighted, which will further improve our ability to establish the causality and hierarchy of chromatin dynamics on genome regulation. |
| Databáze: | OpenAIRE |
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