Integrated nucleic acid testing system to enable TB diagnosis in peripheral settings
| Autor: | Pranav Kumar, Hsiang-Wei Lu, Gerard A. Cangelosi, Ryan P. Talbot, David S. Boyle, Masahiro Narita, Rama Murthy Sakamuri, Robert Doebler, Kris M. Weigel, Keith Herrington, Tanya M. Ferguson, Felicia K. Nguyen, Angelika Niemz |
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| Rok vydání: | 2020 |
| Předmět: |
Tuberculosis
Biomedical Engineering Bioengineering Sensitivity and Specificity Biochemistry Article Mycobacterium tuberculosis Cartridge Nucleic Acids medicine Humans Detection limit Chromatography biology business.industry Sputum General Chemistry Amplicon medicine.disease biology.organism_classification DNA extraction Nucleic acid medicine.symptom business Nucleic Acid Amplification Techniques |
| Zdroj: | Lab Chip |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/d0lc00445f |
| Popis: | To facilitate treatment and limit transmission of tuberculosis (TB), new methods are needed to enable rapid and affordable diagnosis of the disease in high-burden low-resource settings. We have developed a prototype integrated nucleic acid testing device to detect Mycobacterium tuberculosis (M.tb) in sputum. The device consists of a disposable cartridge and compact, inexpensive instrument that automates pathogen lysis, nucleic acid extraction, isothermal DNA amplification and lateral flow detection. A liquefied and disinfected sputum sample is manually injected into the cartridge, and all other steps are automated, with a result provided in |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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