Presence of Acylated Homoserine Lactones (AHLs) and AHL-Producing Bacteria in Meat and Potential Role of AHL in Spoilage of Meat
| Autor: | Kristian Fog Nielsen, Jesper Bartholin Bruhn, Allan Beck Christensen, Lone Gram, Lars Flodgaard, Michael Givskov, Thomas Ostenfeld Larsen |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Meat
medicine.medical_treatment Food spoilage Homoserine Applied Microbiology and Biotechnology Serratia proteamaculans Mass Spectrometry Microbiology chemistry.chemical_compound 4-Butyrolactone Meat spoilage medicine Animals Protease Bacteria Ecology biology Wild type food and beverages Hafnia alvei biochemical phenomena metabolism and nutrition biology.organism_classification Enterobacteriaceae Phenotype chemistry Food Microbiology Chromatography Thin Layer Chromatography Liquid Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 70:4293-4302 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.70.7.4293-4302.2004 |
| Popis: | Quorum-sensing (QS) signals ( N -acyl homoserine lactones [AHLs]) were extracted and detected from five commercially produced vacuum-packed meat samples. Ninety-six AHL-producing bacteria were isolated, and 92 were identified as Enterobacteriaceae. Hafnia alvei was the most commonly identified AHL-producing bacterium. Thin-layer chromatographic profiles of supernatants from six H. alvei isolates and of extracts from spoiling meat revealed that the major AHL species had an R f value and shape similar to N -3-oxo-hexanoyl homoserine lactone (OHHL). Liquid chromatography-mass spectrometry (MS) (high-resolution MS) analysis confirmed the presence of OHHL in pure cultures of H. alvei . Vacuum-packed meat spoiled at the same rate when inoculated with the H. alvei wild type compared to a corresponding AHL-lacking mutant. Addition of specific QS inhibitors to the AHL-producing H. alvei inoculated in meat or to naturally contaminated meat did not influence the spoilage of vacuum-packed meat. An extracellular protein of approximately 20 kDa produced by the H. alvei wild-type was not produced by the AHL-negative mutant but was restored in the mutant when complemented by OHHL, thus indicating that AHLs do have a regulatory role in H. alvei . Coinoculation of H. alvei wild-type with an AHL-deficient Serratia proteamaculans B5a, in which protease secretion is QS regulated, caused spoilage of liquid milk. By contrast, coinoculation of AHL-negative strains of H. alvei and S. proteamaculans B5a did not cause spoilage. In conclusion, AHL and AHL-producing bacteria are present in vacuum-packed meat during storage and spoilage, but AHL does not appear to influence the spoilage of this particular type of conserved meat. Our data indicate that AHL-producing H. alvei may induce food quality-relevant phenotypes in other bacterial species in the same environment. H. alvei may thus influence spoilage of food products in which Enterobacteriaceae participate in the spoilage process. |
| Databáze: | OpenAIRE |
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