Dengue Virus Infects Macrophages and Dendritic Cells in a Mouse Model of Infection
| Autor: | P. Robert Beatty, Eva Harris, Jennifer L. Kyle |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Time Factors
viruses Viral pathogenesis Secondary infection Dengue virus Antibodies Viral Virus Replication medicine.disease_cause Tropism Microbiology Dengue fever Dengue Mice medicine Animals Immunology and Allergy Receptors Interferon biology Reverse Transcriptase Polymerase Chain Reaction Macrophages virus diseases Dendritic Cells Dendritic cell Dengue Virus Viral Load biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Virology Disease Models Animal Flavivirus Infectious Diseases RNA Viral Cellular Tropism |
| Zdroj: | The Journal of Infectious Diseases. 195:1808-1817 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/518007 |
| Popis: | Dengue fever is a mosquitoborne viral illness caused by 4 dengue viruses (DENV-1-4). The cellular tropism of DENV has not been definitively determined, despite its importance for understanding viral pathogenesis and identifying therapeutic targets. To define DENV cellular tropism in a small animal model, 129/Pas mice lacking interferon-alpha/beta and/or-gamma receptors were infected with DENV via a subcutaneous route. During the first week after infection, virus was present in lymph nodes, spleen, bone marrow, and circulating white blood cells. F4/80+CD11b+ macrophages and CD11c+ dendritic cells were demonstrated to be targets for DENV-2 infection in the spleen by flow cytometry directed to structural and nonstructural DENV proteins and by magnetic bead separation followed by strand-specific reverse-transcriptase polymerase chain reaction. We find that the initial cellular tropism of DENV in mice is similar to that reported in humans, thereby paving the way for investigation of cellular tropism and pathogenesis of DENV in primary and secondary infections. |
| Databáze: | OpenAIRE |
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