Inhibitory effect of α-amyrin acetate isolated from Fraxinus rhynchophylla on Th17 polarization
| Autor: | Sunggun Kim, Kwang Woo Hwang, So-Young Park, Jae-Sun Lee, Da Kyung Lee, Na Yeon Kim, Dae-Gun Seo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male STAT3 Transcription Factor Amyrin medicine.medical_treatment Retinoic acid Pharmaceutical Science 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot RAR-related orphan receptor gamma Drug Discovery medicine Animals Oleanolic Acid STAT3 030304 developmental biology Pharmacology 0303 health sciences medicine.diagnostic_test biology Plant Extracts Interleukin-17 Interleukin Cell Polarity Cell Differentiation Nuclear Receptor Subfamily 1 Group F Member 3 Molecular biology Mice Inbred C57BL Cytokine Complementary and alternative medicine chemistry Fraxinus 030220 oncology & carcinogenesis biology.protein Molecular Medicine Th17 Cells Interleukin 17 |
| Zdroj: | Phytomedicine : international journal of phytotherapy and phytopharmacology. 63 |
| ISSN: | 1618-095X |
| Popis: | Background T helper 17 (Th17) cells, which are differentiated from CD4+ T cells, drive inflammation, leading to autoimmune diseases such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease. Therefore, inhibiting Th17 polarization could be a therapeutic target for inflammatory diseases. Purpose We investigated the inhibitory effect of Fraxinus rhynchophylla (Oleaceae) on Th17 differentiation and found its active component. Study design The activity of F. rhynchophylla and its active constituent was verified using CD4+ cells extracted from C57BL/6 mice. Methods Micro-environment for Th17 polarization was provided to CD4+ cells and the effect of treatment with samples was measured by enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and Western blot. Results The extract of F. rhynchophylla Hance and its chemical constituent, α-amyrin acetate, which was isolated via bioassay-guided isolation, significantly inhibited Th17 polarization as revealed when interleukin (IL)-17, a characteristic cytokine produced by Th17 cells, was measured. Furthermore, the inhibitory effect of α-amyrin acetate was compared to the amyrin derivatives, α-amyrin and β-amyrin. All displayed a suppressive effect on Th17 polarization and all reduced the expression of single transducer and activator of transcription 3 (STAT3) and retinoic acid receptor-related orphan receptor γt (RORγt), which are crucial transcription factors regulating Th17 differentiation. α-Amyrin acetate, however, exhibited the most prominent effects, which indicates that the functional group, acetate, might strengthen the inhibitory effect on Th17 differentiation. Conclusion Taken together, these results suggest that the extract of F. rhynchophylla and its active constituent, α-amyrin acetate, could be applied as a potential therapeutic agent for autoimmune disorders such as rheumatoid arthritis. |
| Databáze: | OpenAIRE |
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