Identification of Type III Secretion Inhibitors for Plant Disease Management
Autor: | Pau Sebastià, Roger de Pedro Jové, Marc Valls |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Ralstonia solanacearum biology Bacterial wilt fungi food and beverages Virulence 010501 environmental sciences Bacterial growth biology.organism_classification 01 natural sciences Plant disease Microbiology Type three secretion system 03 medical and health sciences 030104 developmental biology Secretion Organism 0105 earth and related environmental sciences |
Zdroj: | Methods in Molecular Biology ISBN: 9781071609538 Digital.CSIC. Repositorio Institucional del CSIC instname |
Popis: | Bacterial plant pathogens are among the most devastating threats to agriculture. To date, there are no effective means to control bacterial plant diseases due to the restrictions in the use of antibiotics in agriculture. A novel strategy under study is the use of chemical compounds that inhibit the expression of key bacterial virulence determinants. The type III secretion system is essential for virulence of many Gram-negative bacteria because it injects into the plant host cells bacterial proteins that interfere with their immune system. Here, we describe the methodology to identify bacterial type III secretion inhibitors, including a series of protocols that combine in planta and in vitro experiments. We use Ralstonia solanacearum as a model because of the number of genetic tools available in this organism and because it causes bacterial wilt, one of the most threatening plant diseases worldwide. The procedures presented can be used to evaluate the effect of different chemical compounds on bacterial growth and virulence. |
Databáze: | OpenAIRE |
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