ULTRASOUND-ASSISTED MAGNETIC NANOPARTICLE-BASED GENE DELIVERY
| Autor: | Zhixiang Wang, Oleksandra Savchenko, Jie Chen, Wei Zhang, Gaser N. Abdelrasoul, Abdalla Abdrabou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Nanoparticle
02 engineering and technology Paramagnetism Spectrum Analysis Techniques Nanotechnology Polyethyleneimine Magnetite Nanoparticles Materials 0303 health sciences Multidisciplinary medicine.diagnostic_test Chemistry Physics Magnetism Gene Transfer Techniques Transfection Condensed Matter Physics Flow Cytometry 021001 nanoscience & nanotechnology Ultrasonic Waves Spectrophotometry Physical Sciences Magnets Engineering and Technology Medicine Cytophotometry 0210 nano-technology Research Article Materials science Imaging Techniques Cell Survival Science Materials Science Gene delivery Research and Analysis Methods Flow cytometry Gene Delivery 03 medical and health sciences Fluorescence Imaging Gene Expression and Vector Techniques Genetics medicine Humans Viability assay Molecular Biology Techniques Molecular Biology 030304 developmental biology Molecular Biology Assays and Analysis Techniques HEK 293 cells Biology and Life Sciences Magnetic Fields HEK293 Cells Permeability (electromagnetism) Lipofectamine Nanoparticles Magnetic nanoparticles Biomedical engineering |
| Zdroj: | PLoS ONE, Vol 15, Iss 9, p e0239633 (2020) PLoS ONE |
| DOI: | 10.1101/2020.03.31.018440 |
| Popis: | Low-intensity pulsed ultrasound (LIPUS), a special type of ultrasonic stimulation, is attracting a lot of attention for both clinical and scientific research. In this paper, we report a concept of a new method using magnetic nanoparticles (MNPs) for LIPUS-assisted gene delivery. The MNPs are iron oxide superparamagnetic nanoparticles, coated with polyethyleneimine (PEI), which introduces a high positive surface charge, favorable for the binding of genetic material. Due to the paramagnetic properties of the MNPs, the application of an external magnetic field increases transfection efficiency; meanwhile, LIPUS stimulation enhances cell permeability. We found out that stimulation at the intensity of 30 mW/cm2 for 10 minutes yields optimal results with a minimal adverse effect on the cells. Combining the effect of the external magnetic field and LIPUS, the genetic material (GFP or Cherry Red plasmid in our case) can enter the cells. The flow cytometry results showed that by using just a magnetic field to direct the genetic material, the transfection efficiency of HEK 293 cells that were treated with our MNPs was 56.1%. Coupled with LIPUS stimulation, it increased to 61.5% or 19% higher than the positive control (Lipofectamine 2000). In addition, compared with the positive control, our method showed less toxicity. Cell viability after transfection was 63.61%, 19% higher than with the standard transfection technique. In conclusion, we designed a new gene-delivery technique that is affordable, targeted, shows low-toxicity, yet high transfection efficiency, compared to other conventional approaches.The Graphical Abstract |
| Databáze: | OpenAIRE |
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