Influence of melaleuca and copaiba oils onCandida albicansadhesion
| Autor: | Fernanda Mombrini Pigatti, Maria Carolina Martins Mussi, Diego C. P. Rossi, Luiz Antônio de Assis Taveira, Priscila Lie Tobouti, Suzana Cantanhede Orsini Machado de Sousa, Carlos Pelleschi Taborda |
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| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
Antifungal Agents 030106 microbiology Melaleuca Copaiba Oil Microbiology 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration 0302 clinical medicine Candidiasis Oral Candida albicans Copaiba Plant Oils Medicine General Dentistry Stomatitis Dentures biology business.industry Melaleuca alternifolia MICROBIOLOGIA Fabaceae 030206 dentistry biology.organism_classification medicine.disease Stomatitis Denture chemistry Biofilms Sodium hypochlorite Geriatrics and Gerontology business |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0734-0664 |
| DOI: | 10.1111/ger.12172 |
| Popis: | Objective To evaluate the efficacy of Melaleuca alternifolia and Copaifera officinalis in inhibiting the adhesion of Candida albicans biofilm. Background Over 65% of denture wearers suffer from denture stomatitis, which is one of the most prevalent forms of oral candidiasis. This disease is characterised by the inflammation of the oral mucosa in contact with the contaminated denture. The contaminated denture contributes to the switch of C. albicans from yeast to its pathogenic hyphal form. Candida albicans adheres and colonises the polymethylmethacrylate resin surfaces and thus contributes to the development of denture stomatitis. Materials and methods The minimal inhibitory concentration (MIC) of M. alternifolia and Co. officinalis was assessed by the agar dilution method. Sixty-six thermopolymerised acrylic resin squares were used and treated with phosphate-buffered saline, sodium hypochlorite 1%, melaleuca 0.75%, melaleuca 0.375%, melaleuca 0.188% and copaiba 10%. For adherence and biofilm formation, the treated squares were placed in six-well tissue culture plates containing 1 × 107 cells/ml of ATCC1023 or SC5314 in Roswell Park Memorial Institute (RPMI) medium, and after 12 h, the planktonic cells were counted. Results Copaiba oil did not inhibit C. albicans growth. However, melaleuca oil showed an MIC value of 0.375% (3.4 mg/ml) for ATCC10231 and 0.093% (0.84 mg/ml) for SC5314. Conclusions Our results demonstrated that M. alternifolia oil inhibited the growth of C. albicans. Moreover, both oils promoted significant adhesion reduction in the tested strains. These findings suggest the possibility of using these oils in prophylaxes against candidiasis. |
| Databáze: | OpenAIRE |
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