Diagnostic use of recombinant Tha p 2 in the allergy to Thaumetopoea pityocampa
| Autor: | Ana I. Rodriguez-Mahillo, Noelia Carballeda-Sangiao, José Carlos García-Ortiz, Ignacio Moneo, José María Vega, Miguel González-Muñoz, Alain Roques |
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| Přispěvatelé: | Instituto de Salud Carlos III [Madrid] (ISC), Hospital Universitario Río Hortega, Partenaires INRAE, Unité de recherche Zoologie Forestière (URZF), Institut National de la Recherche Agronomique (INRA), Institute of Health Carlos III, URTICLIM, a French project -'Agence Nationale de la Recherche' [ANR-07BDIV-013] |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Allergy [SDV]Life Sciences [q-bio] Immunology Thaumetopoea pityocampa pine processionary caterpillar Enzyme-Linked Immunosorbent Assay Moths human health In vivo tests law.invention law Epidemiology medicine Hypersensitivity Immunology and Allergy Animals Humans Short latency business.industry Allergens medicine.disease pine processionary moth allergy Dermatology Recombinant Proteins 3. Good health setae Clinical diagnosis Case-Control Studies Recombinant DNA Insect Proteins epidemiology business Whole body recombinant allergen |
| Zdroj: | Allergy Allergy, Wiley, 2015, 70 (10), pp.1332-1335. ⟨10.1111/all.12678⟩ |
| ISSN: | 0105-4538 1398-9995 |
| DOI: | 10.1111/all.12678⟩ |
| Popis: | International audience; Thaumetopoea pityocampa causes allergies and skin and ocular lesions. No commercial tools are currently available for the clinical diagnosis of this allergy. We aimed to develop an in vitro method for the diagnosis of this allergy to avoid patients undergoing in vivo tests with insect extracts. Recombinant Tha p 2 was produced and used in an ELISA validated with 15 allergic patients. Subsequently, 42 subjects recruited from a random sampling cross-sectional study were analysed. The ELISA sensitivity and specificity were 93.3% and 100%, respectively, for the allergic patients and 71.4% and 95.3%, respectively, for the epidemiological study. The positive ELISA results correlated with the skin prick test areas with the whole body and the setae extracts. Professional exposure and short latency of symptoms onset were risk factors for a positive result in the ELISA. In conclusion, our ELISA is very useful for T. pityocampa allergy diagnosis and for epidemiologic testing. |
| Databáze: | OpenAIRE |
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