New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis
| Autor: | Aarti Narkhede, Bhagyashri Nagarkar, Abhay Harsulkar, Pallavi Nirmal, Suresh Jagtap |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cartilage Articular medicine.medical_specialty Collagenase induced osteoarthritis Sida cordifolia Osteoarthritis Pharmacology Ginger Protective Agents 03 medical and health sciences 0302 clinical medicine Diclofenac Oral administration Synovium medicine Animals Humans Collagenases Rats Wistar Malvaceae Glycosaminoglycans 030203 arthritis & rheumatology biology business.industry Plant Extracts Cartilage Therapeutic effect General Medicine biology.organism_classification medicine.disease Surgery Rats 030104 developmental biology medicine.anatomical_structure C-Reactive Protein Complementary and alternative medicine Matrix Metalloproteinase 9 Formulation Collagenase Alkaline phosphatase Female Matrix Metalloproteinase 3 business medicine.drug Phytotherapy Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| Popis: | Background Prevalence of osteoarthritis (OA) is on rise on the global scale. At present there are no satisfactory pharmacological agents for treating OA. Our previous study showed that Sida cordifolia L. and Zingiber officinale Rosc. had protective effect on cartilage. Here, we describe the effect of OA-F2, a herbal formulation prepared using combination of these two plants in alleviating OA associated symptoms in a rat model of collagenase-induced OA. Methods OA was induced by intra-articular injection of collagenase type II in wistar rats. Diclofenac (10 mg/kg) was used as a reference control. Rats (n = 6) were divided into 6 groups: Healthy control (HC), osteoarthritic control (OAC), diclofenac (DICLO), OA-F2L (135 mg/kg), OA-F2M (270 mg/kg) and OA-F2H (540 mg/kg). The effects of the 20 days treatment were monitored by parameters like knee diameter, paw volume, paw retraction; serum C-reactive protein (CRP), alkaline phosphatase (ALP) and glycosaminoglycan (GAG). Radiography and histopathology of knee joint were also studied. Additionally, gene expression was studied from isolated synovium tissue proving anti-osteoarthritic potential of OA-F2. Results Oral administration of OA-F2 has significantly prevented knee swelling compared to OAC; OA-F2 and DICLO, significantly reduced paw volume compared to OAC. Paw latency was remarkably increased by OA-F2 compared to OAC. OA-F2L (−0.670, p |
| Databáze: | OpenAIRE |
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