Immune responses elicited by Mycoplasma hyopneumoniae recombinant antigens and DNA constructs with potential for use in vaccination against porcine enzootic pneumonia
| Autor: | Taylor Gonchoroski, Henrique Bunselmeyer Ferreira, Jéssica Andrade Paes, Desirée Cigaran Schuck, Arnaldo Zaha, Veridiana Gomes Virginio |
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| Rok vydání: | 2014 |
| Předmět: |
Swine
Microbiology DNA vaccination law.invention Interferon-gamma Porcine enzootic pneumonia Immune system Bacterial Proteins Mycoplasma hyopneumoniae Antigen law Vaccines DNA Splenocyte Animals HSP70 Heat-Shock Proteins Cells Cultured Antigens Bacterial Immunity Cellular Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Pneumonia of Swine Mycoplasmal Th1 Cells biology.organism_classification Antibodies Bacterial Virology Recombinant Proteins Interleukin-10 Protein Structure Tertiary Vaccination Infectious Diseases Immunoglobulin G Bacterial Vaccines Vaccines Subunit Recombinant DNA Molecular Medicine Female Interleukin-4 Spleen |
| Zdroj: | Vaccine. 32:5832-5838 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2014.08.008 |
| Popis: | Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (PEP) and causes major economic losses to the pig industry worldwide. Commercially available vaccines provide only partial protection and are relatively expensive. In this study, we assessed the humoral and cellular immune responses to three recombinant antigens of M. hyopneumoniae. Immune responses to selected domains of the P46, HSP70 and MnuA antigens (P46102-253, HSP70212-601 and MnuA182-378), delivered as recombinant subunit or DNA vaccines, were evaluated in BALB/c mice. All purified recombinant antigens and two DNA vaccines, pcDNA3.1(+)/HSP70212-601 and pcDNA3.1(+)/MnuA182-378, elicited a strong humoral immune response, indicated by high IgG levels in the serum. The cellular immune response was assessed by detection of IFN-γ, IL-10 and IL-4 in splenocyte culture supernatants. The recombinant subunit and DNA vaccines induced Th1-polarized immune responses, as evidenced by increased levels of IFN-γ. All recombinant subunit vaccines and the pcDNA3.1(+)/MnuA182-378 vaccine also induced the secretion of IL-10, a Th2-type cytokine, in large quantities. The mixed Th1/Th2-type response may elicit an effective immune response against M. hyopneumoniae, suggesting that P46102-253, HSP70212-601 and MnuA182-378 are potential novel and promising targets for the development of vaccines against PEP. |
| Databáze: | OpenAIRE |
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