Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after intracytoplasmic sperm injection
| Autor: | Jennifer G. DeLuca, Giovanna Lazzari, Keith F. DeLuca, Cesare Galli, Elena Ruggeri, Elaine M. Carnevale |
|---|---|
| Přispěvatelé: | Ruggeri, E., Deluca, K.F., Galli, C., Lazzari, G., Deluca, J.G., Carnevale, E.M. |
| Rok vydání: | 2014 |
| Předmět: |
Male
Reproductive immunology Zygote medicine.medical_treatment intracytoplasmic sperm injection Reproductive technology Fertilization in Vitro Biology Oogenesis Intracytoplasmic sperm injection Article Andrology Endocrinology Genetics medicine Animals Horses Sperm Injections Intracytoplasmic Molecular Biology Fertilisation equine oocyte reproductive and urinary physiology Cytoskeleton urogenital system Age Factors Anatomy Cytoskeletal alteration Oocyte Spermatozoa medicine.anatomical_structure Reproductive Medicine Oocytes Animal Science and Zoology Female Folliculogenesis Cell aging Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 27(6) |
| ISSN: | 1031-3613 |
| Popis: | Intracytoplasmic sperm injection (ICSI) is an established method to fertilise equine oocytes, but not all oocytes cleave after ICSI. The aims of the present study were to examine cytoskeleton patterns in oocytes after aging in vitro for 0, 24 or 48 h (Experiment 1) and in potential zygotes that failed to cleave after ICSI of oocytes from donors of different ages (Experiment 2). Cytoplasmic multiasters were observed after oocyte aging for 48 h (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|