The first report of Escherichia fergusonii isolated from non-human primates, in Africa
| Autor: | Moritz Van Vuuren, Barbara Glover, Akinbowale Olajide Jenkins, Jeanette Wentzel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology Wildlife medicine.disease_cause Antimicrobial resistance Microbiology law.invention 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance law medicine Escherichia coli Polymerase chain reaction Non-human primates lcsh:R5-920 biology Public Health Environmental and Occupational Health Escherichia fergusonii Simmons' citrate agar biology.organism_classification Infectious Diseases chemistry Amikacin Colistin lcsh:Medicine (General) Bacteria Research Paper medicine.drug |
| Zdroj: | One Health, Vol 3, Iss C, Pp 70-75 (2017) One Health |
| ISSN: | 2352-7714 |
| Popis: | The aim of this study was to determine the resistance phenotypes of selected enteric bacteria isolated from non-human primates at a wildlife-human interface. Bacterial isolates from faecal samples of non-human primates at two wildlife rehabilitation centres in South Africa were screened for the presence of Escherichia coli . The biochemical characterisation of E. coli and E. coli -like bacteria revealed both adonitol positive and sorbitol negative strains – a unique characteristic of Escherichia fergusonii and Escherichia coli K99. Further tests were carried out to identify the isolates, namely growth on Simmons citrate agar supplemented with 2% adonitol and biochemical tests based on their ability to ferment cellobiose and d -arabitol. Antimicrobial sensitivity was determined with microbroth dilution tests employing microtitre plates with 21 different antimicrobial drugs. Molecular characterisation was done with a duplex polymerase chain reaction (PCR) assay that targeted the yliE and EFER_1569 genes. E. fergusonii strains were confirmed by the presence of a 233 bp segment of the yliE gene and a 432 bp segment of the EFER_1569 gene. Twenty-three E. coli -like bacteria were confirmed as E. fergusonii based on the confirmatory tests and they were in 100% agreement. Approximately 87% of them were resistant to polymyxins B and E (colistin) as well as the carbapenem group with occasional resistance to amikacin. This is the first reported isolation and identification of E. fergusonii strains in non-human primates. The findings point to E. fergusonii as a possible emerging pathogen of zoonotic importance. |
| Databáze: | OpenAIRE |
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