Silymarin protects against radiocontrast-induced nephropathy in mice
| Autor: | Thiago de Melo Costa Pereira, Larissa Zambom Côco, Beatriz Peters, Elisardo C. Vasquez, Bianca P. Campagnaro, Gisele Maziero Alves, Marcella L. Porto, Breno Valentim Nogueira, Silvana S. Meyrelles, Arícia Leone Evangelista Monteiro de Assis, Verônica de Souza Santos |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cell Survival Contrast Media Renal function Apoptosis Pharmacology Kidney Protective Agents urologic and male genital diseases medicine.disease_cause Protein oxidation 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology Silybum marianum Nephropathy Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Milk Thistle General Pharmacology Toxicology and Pharmaceutics Nephritis biology business.industry General Medicine biology.organism_classification medicine.disease Oxidative Stress 030104 developmental biology medicine.anatomical_structure Cystatin C biology.protein business Oxidative stress DNA Damage Silymarin |
| Zdroj: | Life Sciences. 228:305-315 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2019.04.061 |
| Popis: | Silymarin, an extract from Silybum marianum (milk thistle) containing a standardized mixture of flavonolignans that ameliorates some types of liver disease and, more recently, kidney damage, could be used for the ROS-scavenging effect of these antioxidants. Furthermore, contrast-induced nephropathy (CIN) is an iatrogenic impairment of renal function in patients subjected to angiographic procedures for which there is not yet a successful preventative treatment. Recent evidence has shown that this event is related to tubular/vascular injury activated mainly by oxidative stress. However, whether this bioavailable and pharmacologically safe extract protects against CIN is not clear. We proposed to evaluate the possible protective role of the antioxidant silymarin in an experimental model of CIN. Adult male Swiss mice were separated into 6 groups and pretreated orally with silymarin (50, 200 and 300 mg/kg), N-acetylcysteine (200 mg/kg) or vehicle for 5 days before the CIN and control groups. Renal function was analyzed by plasma creatinine, urea and cystatin C levels. Additionally, blood reactive oxygen species (ROS) were evaluated using ROS bioavailability, protein oxidation and DNA damage. Renal oxidative damage was evaluated using apoptosis/cell viability assays and histological analysis. We showed that silymarin preserved renal function and decreased systemic and renal oxidative damage (antigenotoxic and antiapoptotic properties, respectively) in a dose-dependent manner and was superior to conventional treatment with N-acetylcysteine. Histologically, silymarin treatment also had beneficial effects on renal glomerular and tubular injuries. Therefore, silymarin prophylaxis may be an interesting strategy for the prevention of CIN. |
| Databáze: | OpenAIRE |
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