The sulcatone receptor of the strict nectar-feeding mosquito Toxorhynchites amboinensis
| Autor: | Esther Yakir, Jonathan D. Bohbot, Amir Dekel |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Arthropod Antennae
0106 biological sciences Patch-Clamp Techniques Xenopus DEET Zoology Context (language use) Olfaction Aedes aegypti Biology Receptors Odorant 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Animals Molecular Biology 030304 developmental biology Aedes 0303 health sciences fungi Ketones biology.organism_classification Attraction Electrophysiological Phenomena Chemical ecology 010602 entomology Culicidae chemistry Insect Repellents Insect Science Oocytes Insect repellent |
| Zdroj: | Insect Biochemistry and Molecular Biology. 111:103174 |
| ISSN: | 0965-1748 |
| Popis: | Controlling Ae. aegypti populations and the prevention of mosquito bites includes the development of monitoring, repelling and attract-and-kill strategies that are based on understanding the chemical ecology of these pests. Olfactory-mediated attraction to mammals has recently been linked to the mosquito Aedes aegypti odorant receptor Or4, which is activated by animal-released 6-Methyl-5-hepten-2-one (sulcatone). This odorant is also a major component of flower scents and may play a role outside animal-host seeking. To explore the role of this chemical cue, we looked at the interaction between sulcatone and an Or4 homolog expressed in the antennae of the strict nectar-feeding mosquito Toxorhynchites amboinensis. Using the two-electrode voltage clamp of Xenopus oocytes as a heterologous expression system, we show that this receptor is a high intensity sulcatone receptor comparable to its Aedes counterparts. We also show that OR4 is activated by other aliphatic ketones and is inhibited by DEET. This pharmacological characterization suggests that sulcatone may be operating in more than one context in the Culicidae family. |
| Databáze: | OpenAIRE |
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