Detection of K-rasPoint Mutations at Codon 12 in Pancreatic Juice for the Diagnosis of Pancreatic Cancer by Hybridization Protection Assay: A Simple Method for the Determination of the Types of Point Mutations
| Autor: | Hideki Ohta, Yoshiharu Motoo, Tadashi Yoshimura, Chieko Miyagi, Yasuhiro Tsuji, Takashi Okai, Yoshitake Satomura, Hiroyuki Watanabe, Norio Sawabu, Yasushi Yamaguchi |
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| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Hybridization Protection Assay Pancreatic disease Molecular Sequence Data Biology medicine.disease_cause Polymerase Chain Reaction Article Pancreatic Juice Pancreatic cancer medicine Humans Point Mutation Codon Genetics Mutation Base Sequence Hybridization probe Point mutation K‐ras mutation medicine.disease Molecular biology Pancreatic Neoplasms Genes ras medicine.anatomical_structure Acridinium ester Oncology Genetic diagnosis Pancreatic juice Hybridization protection assay DNA Probes Pancreas |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 0910-5050 |
| Popis: | The present study was undertaken to detect K‐ras oncogene point mutations at codon 12 in pure pancreatic juice (PPJ) by the hybridization protection assay (HPA) method for the diagnosis of pancreatic cancer (PC). This assay can be carried out within 30 min and can determine not only the presence of a mutation, but also the mutational type of K‐ras at codon 12. The minimal ratio of mutant DNA detectable by the HPA was 5–10% of the total DNA. PPJ was collected through a cannula under duodenal fiberscope control from 20 patients with PC and 20 patients with chronic pancreatitis (CP). Analysis of PPJ by the HPA revealed that the incidence of K‐ras point mutations at codon 12 was 55% (11/20) in patients with PC and 0% (0/20) in those with CP. Mutational types of K‐ras at codon 12 in PC were aspartic acid (Asp) in nine cases, both Asp and cysteine in one case, and arginine in one case. Analysis of K‐ras point mutations at codon 12 in PPJ using the HPA method seems promising as a new genetic test for the diagnosis of PC, because the HPA method is simple, and can easily determine the mutational type. |
| Databáze: | OpenAIRE |
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