Rapid differentiation between livestock-associated and livestock-independent Staphylococcus aureus CC398 clades
| Autor: | Robert Skov, Anders Rhod Larsen, Frédéric Laurent, Judy Natalia Jiménez, Andreas Petersen, Alex van Belkum, Jesper Larsen, Lance Price, Paal S. Andersen, Katerina Soldanova, Stien Vandendriessche, Caterina Mammina, Saara Salmenlinna, Maliha Aziz, Marc Stegger, Tania Contente-Cuomo, Cindy M. Liu |
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| Přispěvatelé: | Stegger, M, Liu, CM, Larsen, J, Soldanova, K, Aziz, M, Contente-Cuomo, T, Petersen, A, Vandendriessche, S, Jimenez, JN, Mammina, C, van Belkum, A, Salmenlinna, S, Laurent, F, Skov, RL, Larsen, AR, Andersen, PS, Price LB |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Staphylococcus aureus
Livestock Psychologie appliquée lcsh:Medicine Biology Settore MED/42 - Igiene Generale E Applicata medicine.disease_cause Staphylococcal infections Polymorphism Single Nucleotide Animal Diseases Microbiology law.invention 03 medical and health sciences Phylogenetics law medicine Animals Humans MRSA ST398 clades differentiation Clade lcsh:Science Phylogeny Polymerase chain reaction 030304 developmental biology 0303 health sciences Multidisciplinary Phylogenetic tree 030306 microbiology lcsh:R Staphylococcal Infections Sciences bio-médicales et agricoles medicine.disease Methicillin-resistant Staphylococcus aureus 3. Good health Genes Bacterial lcsh:Q Mobile genetic elements Biologie Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 11, p e79645 (2013) PloS one, 8 (11 PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Staphylococcus aureus clonal complex 398 (CC398) isolates cluster into two distinct phylogenetic clades based on single-nucleotide polymorphisms (SNPs) revealing a basal human clade and a more derived livestock clade. The scn and tet(M) genes are strongly associated with the human and the livestock clade, respectively, due to loss and acquisition of mobile genetic elements. We present canonical single-nucleotide polymorphism (canSNP) assays that differentiate the two major host-associated S. aureus CC398 clades and a duplex PCR assay for detection of scn and tet (M). The canSNP assays correctly placed 88 S. aureus CC398 isolates from a reference collection into the human and livestock clades and the duplex PCR assay correctly identified scn and tet(M). The assays were successfully applied to a geographically diverse collection of 272 human S. aureus CC398 isolates. The simple assays described here generate signals comparable to a whole-genome phylogeny for major clade assignment and are easily integrated into S. aureus CC398 surveillance programs and epidemiological studies. © 2013 Stegger et al. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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