Conversion of Human Fibroblasts into Induced Neural Stem Cells by Small Molecules
| Autor: | Donghui Liu, Grigori Rychkov, Plinio Hurtado, Hai-Yun Luo, Tao Zhang, Larisa Bobrovskaya, Xin-Fu Zhou |
|---|---|
| Přispěvatelé: | Liu, Donghui, Rychkov, Grigori, Hurtado, Plinio, Luo, Hai Yun, Zhang, Tao, Bobrovskaya, Larisa, Zhou, Xin Fu |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
QH301-705.5
Down-Regulation Catalysis Cell Line Small Molecule Libraries Inorganic Chemistry Mice Neural Stem Cells Animals Humans Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy Neurons human fibroblasts Organic Chemistry small molecules induced neuron stem cells Cell Differentiation General Medicine Fibroblasts Electrophysiological Phenomena Up-Regulation Computer Science Applications Oligodendroglia Chemistry Astrocytes Biomarkers |
| Zdroj: | International Journal of Molecular Sciences, Vol 23, Iss 1740, p 1740 (2022) International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1740 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Induced neural stem cells (iNSCs) reprogrammed from somatic cells hold great potentials for drug discovery, disease modelling and the treatment of neurological diseases. Although studies have shown that human somatic cells can be converted into iNSCs by introducing transcription factors, these iNSCs are unlikely to be used for clinical application due to the safety concern of using exogenous genes and viral transduction vectors. Here, we report the successful conversion of human fibroblasts into iNSCs using a cocktail of small molecules. Furthermore, our results demonstrate that these human iNSCs (hiNSCs) have similar gene expression profiles to bona fide NSCs, can proliferate, and are capable of differentiating into glial cells and functional neurons. This study collectively describes a novel approach based on small molecules to produce hiNSCs from human fibroblasts, which may be useful for both research and therapeutic purposes. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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