Long-chain polyamines (oligoamines) exhibit strong cytotoxicities against human prostate cancer cells
| Autor: | Venodhar K. Reddy, Benjamin Frydman, Aparajita Sarkar, Hirak S. Basu, Laurence J. Marton, Andrei V. Blokhin, Aldonia Valasinas, Subhra Bhattacharya |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Time Factors Cell Survival Stereochemistry Clinical Biochemistry Pharmaceutical Science Antineoplastic Agents Biochemistry Structure-Activity Relationship chemistry.chemical_compound Cell Line Tumor Drug Discovery LNCaP Humans MTT assay Cytotoxicity Molecular Biology Dose-Response Relationship Drug Propylamines Chemistry Organic Chemistry Prostatic Neoplasms Stereoisomerism DNA In vitro Cell culture Cancer cell Molecular Medicine Drug Screening Assays Antitumor Polyamine Cell Division |
| Zdroj: | Bioorganic & Medicinal Chemistry. 11:4121-4131 |
| ISSN: | 0968-0896 |
| Popis: | α N , ω N -bis(ethyl) octamine SL-11160, decamine SL-11159, dodecamine SL-11226, and tetradecamine SL-11175 were chemically synthesized. We called this class of compounds ‘oligoamines’. In these compounds, each –NH 2 + residue is separated by four CH 2 residues. trans -Unsaturation was also introduced into the center of the oligoamine chain resulting in the trans -octamine SL-11158, trans -decamine SL-11144, trans -dodecamine SL-11172 and trans -tetradecamine SL-11227. cis -Unsaturation gave the cis -octamine SL-11157 and cis -decamine SL-11150. When assayed for their growth inhibitory effect against four human prostate cancer cell lines LnCap, DU-145, DuPro, and PC-3 by a MTT assay, the ID 50 values were less than 1 μM in all four cell lines. On day 6 of treatment, 2 μM SL-11159, SL-11144 and SL-11175 killed over five logs of DuPro cells while SL-11172 killed over four logs as determined by a colony forming efficiency (CFE) assay. In addition, SL-11159, SL-11226 and SL-11227 killed four logs of PC-3 cells. PC-3 cells are generally resistant to shorter chain polyamine analogues. Such a level of cytotoxicity in any of the prostate tumor cell lines has not been observed for any other polyamine analogues tested thus far. The DU-145 cell line was too sensitive to oligoamines to perform a CFE analysis and the DuPro cell line was too sensitive to SL-11227 treatment to obtain reproducible CFE data. Interestingly, all 10 oligoamines were efficient DNA aggregators in a cell-free system and their cytotoxicities generally parallel their capacities to aggregate DNA. |
| Databáze: | OpenAIRE |
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