Deregulated expression of E2F-1 induces cyclin A- and E-associated kinase activities independently from cell cycle position
| Autor: | Thomas Soucek, Markus Hengstschläger, Peter D. Adams, Oliver Pusch, Elke Hengstschläger-Ottnad |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Cyclin E Cyclin D Cyclin A Cyclin B Cell Cycle Proteins Cell Line Cyclin-dependent kinase Cyclins Genetics Animals RNA Messenger Molecular Biology Cyclin-dependent kinase 1 biology Cell Cycle Molecular biology Cyclin-Dependent Kinases E2F Transcription Factors Rats DNA-Binding Proteins Gene Expression Regulation biology.protein Cyclin-dependent kinase complex Carrier Proteins Transcription Factor DP1 E2F1 Transcription Factor Cyclin A2 Retinoblastoma-Binding Protein 1 Transcription Factors |
| Zdroj: | Oncogene. 14:2251-2257 |
| ISSN: | 1476-5594 0950-9232 |
| Popis: | The transcription factor E2F activates genes required for S phase, such as cyclin E and cyclin A. We show that, contrary to long term effects of E2F-1 overexpression, short ectopic overexpression of this transcription factor in logarithmically growing cells does neither affect the cell cycle distribution nor the cell size, but heavily induces cyclin E and A expression as well as cyclin E- and A-dependent kinase activities. We further separated logarithmically growing E2F-1-overexpressing cells according to their different cell cycle phases by centrifugal elutriation. These experiments revealed that deregulated E2F-1 expression triggers high levels of cyclin E and A expression and kinase activities in small early G1 cells, normally not exhibiting these activities. These effects on the regulation of cyclin E- and A-associated kinases are not accompanied by any detectable alteration in the rate of progression through the cell cycle, suggesting that these changes are independent of any mitogenic properties of E2F-1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|