A quantitative RT-PCR assay for rapid detection of Eurasian-lineage H10 subtype influenza A virus
| Autor: | Thomas J. DeLiberto, Xiu-Feng Wan, Yi Zhang, Hailiang Sun, Yifei Xu, Lanny W. Pace, John A. Baroch, Jianli Xue, Guoliang Hu, Elizabeth Bailey |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Lineage (genetic) 030106 microbiology Immunology Biology medicine.disease_cause Virology Rapid detection Article 03 medical and health sciences 030104 developmental biology Medical microbiology Real-time polymerase chain reaction medicine Influenza A virus Molecular Medicine |
| Zdroj: | Virologica Sinica. 31:444-447 |
| ISSN: | 1995-820X 1674-0769 |
| Popis: | Quantitative RT-PCR (qRT-PCR) has been commonly used for IAV detection in influenza surveillance and disease diagnosis because of its high sensitivity, specificity, and high throughput. A number of HA subtype specific qRT-PCR methods have been developed, including H5, H7, and H9 (Monne et al., 2008). However, there is still lack of a specific qRT-PCR method for detecting the emerging H10N8 IAVs. In this study, a Eurasian-lineage H10 specific qRT-PCR is developed and validated. |
| Databáze: | OpenAIRE |
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