Effect of Parachlamydia acanthamoebae on pulmonary function parameters in a bovine respiratory model
| Autor: | Markus Lohr, Gilbert Greub, Carola Ostermann, Petra Reinhold, Roland Diller, Annette Prohl |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty 030106 microbiology Cattle Diseases Respiratory physiology Pulmonary function testing 03 medical and health sciences Medicine Animals Prospective Studies Respiratory system Lung Tidal volume Acid-Base Equilibrium Chlamydiales General Veterinary business.industry Pulmonary Gas Exchange Respiration Respiratory infection Respiratory Function Tests Disease Models Animal medicine.anatomical_structure Anesthesia Breathing Respiratory Mechanics Animal Science and Zoology Cattle business Gram-Negative Bacterial Infections Pulmonary Ventilation Respiratory minute volume |
| Zdroj: | Veterinary journal (London, England : 1997). 213 |
| ISSN: | 1532-2971 |
| Popis: | The aim of this study was to evaluate pulmonary dysfunction induced by experimental infection with Parachlamydia acanthamoebae in calves. Intra-bronchial inoculation with P. acanthamoebae was performed in 31 calves aged 2–3 months old at two different challenge doses of 108 and 1010 inclusion-forming units (IFU) per animal. Control animals received heat-inactivated bacteria. The effects on pulmonary gas exchange were determined by arterial blood gas analysis and haemoximetry during the 7 days post inoculation (DPI). For pulmonary function testing (PFT), impulse oscillometry, capnography, and measurement of O2 uptake were undertaken in spontaneously breathing animals 7 and 3 days before inoculation, and were repeated until 10 DPI. In the early phase after challenge (1–3 DPI), mild hypoxaemia occurred, which was accompanied by a significant reduction in both tidal volume and alveolar volume (each related to bodyweight, BW). In parallel, expiratory flow rate and specific ventilation (i.e. minute ventilation related to O2 uptake) were significantly increased. Minute ventilation and alveolar ventilation (each related to metabolic BW) increased significantly, due to higher respiratory rates, lasting until 4 and 5 DPI, respectively. Oxygen uptake was slightly reduced during the first 2 days after challenge, but increased significantly during the recovery phase, from 4 to 8 DPI. No deterioration in respiratory mechanics or acid-base balance was observed. Respiratory infection with 1010 IFU P. acanthamoebae per calf induced mild respiratory dysfunction, mainly characterised by hypoxaemia. The study findings do not indicate severe pathophysiological consequences of P. acanthamoebae infection on pulmonary function in the bovine host. |
| Databáze: | OpenAIRE |
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