Rapid, label-free detection of cerebral ischemia in rats using hyperspectral imaging
| Autor: | Shi Wang, Zhao Li, Tunan Chen, Hua Feng, Kang Ma, Haifeng Wang, Chuhua Fu, Fei Li, Chuanyan Yang, Lu Zhao, Sheng Gong, Ning Mu, Dayong Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Ischemia H&E stain Brain Ischemia Brain ischemia Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine In vivo Internal medicine medicine Animals Stroke Ischemic Stroke business.industry General Neuroscience Hyperspectral imaging Hyperspectral Imaging medicine.disease Rats Disease Models Animal 030104 developmental biology Cardiology business Perfusion 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | Journal of neuroscience methods. 329 |
| ISSN: | 1872-678X |
| Popis: | Background Stroke is the third most common cause of disability and the second most common cause of death worldwide. Ischemia, one of the two broad categories of stroke, is characterized by a lack of sufficient amounts of blood in order to supply an adequate amount of oxygen and nutrients. It is important to assess the part of the brain that becomes ischemic and necrotic during neurosurgery or experiments in real time. However, there is currently no effective means to achieve this goal. New method We proposed a method based on hyperspectral imaging (HSI) for the real-time detection of a varied range of ischemic brain tissues in vivo or ex vivo and assessed the practical utility of a model of ischemic stroke in rats. Results The results showed that hyperspectral images processed with a ratio of spectral reflectance at 545 and 560 nm (R545/R560) could identify early brain ischemia and accurately show regions of ischemia. Comparison with existing methods We verified the area imaged by HSI using hematoxylin and eosin (HE) and 2, 3, 5-triphenyltetrazolium chloride (TTC) staining methods. This technique could precisely image the ischemic part of the brain in vivo and ex vivo. Conclusions These results demonstrate the practical utility of HSI for the real-time detection of cerebral ischemia in rats. By providing rapid assessment of brain tissue perfusion, HSI may help doctors recognize ischemic regions quickly and precisely during surgery as well as have great utility in the experimental process. |
| Databáze: | OpenAIRE |
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